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| WBPicture0000013927 | Description | Figure 8. Biopterin synthetic genes are expressed in biogenic amine neurons and in the epidermis. (A) Expression of cat-4 GFP reporters in larval stage 2 (L2) worms, construct with 2.7 kb upstream sequence (strain CZ9719). Top: Superficial focal plane showing epidermal expression, especially in the hyp7 syncytium. Seam cells have undergone doubling division and can be seen along the lateral side as darker regions among the brightly staining hyp7 cell. Dendritic endings of CEP neurons can be seen at the tip of the 'nose.' Middle: Medial focal plane showing anal cells, strongly expressing tail epidermal cells and posterior intestinal cells expressing GFP. Bottom: Medial focal plane showing epidermal expression in the body and head. In these transgenics, expression in the head is seen in hyp6, but not the more anterior hyp5. NSM and CEP neuron somas are seen in the head, plus some neuronal processes (especially CEP processes). A few other neuronal somas stain less brightly. The anterior intestine also shows GFP expression, as do some rectal epithelial cells (likely B and Y cells). (B) ptps-1-GFP reporters. Left: head showing NSM expression plus other neurons and nonneuronal cells, including anterior intestine (dorsal-ventral view, maximum intensity projection) (MIP); strain OH11619, described by Zhang et al. (2014). Right: vulval region showing expression in neurons VC4, 5 (weak) and HSN, plus lateral seam cells (lateral view, MIP); strain CZ18321. (C) pcbd-1 reporter in larva showing expression in epidermal syncytium hyp7, tail and head epidermis, but not in seam cells; strain CZ19212. Out of focus: pharyngeal muscle and rectal epithelium expression. Inset: enlargement of midbody region. (D) qdpr-1 reporters. Top images: single optical sections from larva showing broad epidermal expression, but little expression in neurons; strain CZ19213. Insets: enlargements of midbody regions. Bottom image: Head showing expression in identified 5HT and DA neurons: NSMs, ADFs, CEPs, and other cells, with reduced epidermal expression; strain CZ19215 (lateral view, MIP). (E) gfrp-1 reporter showing expression in epidermal syncytium hyp7 (top: superficial focal plane) and other epidermal cells (bottom: central focal plane). (A-E) Anterior is to the left in all worms. (A, C, and E) Standard epifluorescence. (B and D) Laser scanning confocal imaging; (B and D, bottom) images are maximal intensity projection of Z-stack; and (D, top and middle) single confocal image planes. | |||
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| Crop | Crop_picture | WBPicture0000013928 | |||
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| Acknowledgment | Template | WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL> | |||
| Publication_year | 2015 | ||||
| Article_URL | DOI | id | 10.1534/genetics.114.174110 | ||
| Journal_URL | Genetics | ||||
| Publisher_URL | GSA | ||||
| Reference | WBPaper00046585 |
