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WBPicture0000013928DescriptionFigure 8. Biopterin synthetic genes are expressed in biogenic amine neurons and in the epidermis. (A) Expression of cat-4 GFP reporters in larval stage 2 (L2) worms, construct with 2.7 kb upstream sequence (strain CZ9719). Top: Superficial focal plane showing epidermal expression, especially in the hyp7 syncytium. Seam cells have undergone doubling division and can be seen along the lateral side as darker regions among the brightly staining hyp7 cell. Dendritic endings of CEP neurons can be seen at the tip of the 'nose'. Middle: Medial focal plane showing anal cells, strongly expressing tail epidermal cells and posterior intestinal cells expressing GFP. Bottom: Medial focal plane showing epidermal expression in the body and head. In these transgenics, expression in the head is seen in hyp6, but not the more anterior hyp5. NSM and CEP neuron somas are seen in the head, plus some neuronal processes (especially CEP processes). A few other neuronal somas stain less brightly. The anterior intestine also shows GFP expression, as do some rectal epithelial cells (likely B and Y cells).
NameFigJ_A.jpg
CropCropped_fromWBPicture0000013927
DepictExpr_patternExpr12165
Anatomy (12)
Life_stageWBls:0000003
WBls:0000023
WBls:0000041
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2015
Article_URLDOIid10.1534/genetics.114.174110
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00046585