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| WBPicture0000014366 | Description | Figure 5 TWK-7 is expressed in ventral nerve cord, head, and tail neurons. (A) Cytosolic DsRed expression driven by the twk-7 promoter is overlaid withthe cholinergic neuron-specific cytosolic GFP expression mediated by the unc-17 promoter. Coexpression is indicated by yellow color. (B) Maximumprojection of a z-series through the head region revealed that DsRed fluorescence is seen in cholinergic and noncholinergic head neurons. (C) Maximum projection of a z-series through the cholinergic region of the ventral nerve cord. Detailed coexpression analyses of TWK-7- and UNC-17-expressing cells in the ventral nerve cord indicate that TWK-7 is present in the cholinergic A-, AS-, and B-type motor neurons as well as in UNC-172 cells. According to their stereotypic positions, these were identified as GABAergic D-type motor neurons. In addition, TWK-7 expression is seen in unidentified tail neurons. (D) Maximum projection of a z-series through the cholinergic region of the ventral nerve cord. Expression of a TWK-7::mCherry fusion protein, which includes the entire TWK-7 channel protein, leads to a punctate subcellular and membrane-associated localization. (E) Coexpression of cytosolic GFP is driven by the cholinergic neuron-specific unc-17 promoter. | |||
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| Name | Fig5.jpg | ||||
| Depict | Expr_pattern | Expr13247 | |||
| Anatomy | WBbt:0005274 | ||||
| WBbt:0005278 | |||||
| WBbt:0005334 | |||||
| WBbt:0005336 | |||||
| WBbt:0005339 | |||||
| WBbt:0005829 | |||||
| WBbt:0006751 | |||||
| WBbt:0006759 | |||||
| Cellular_component | GO:0043025 | ||||
| Acknowledgment | Template | WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL> | |||
| Publication_year | 2016 | ||||
| Article_URL | DOI | id | 10.1534/genetics.116.188896 | ||
| Journal_URL | Genetics | ||||
| Publisher_URL | GSA | ||||
| Reference | WBPaper00049960 |
