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WBPicture0000014940DescriptionFigure 1: Max intensity projections showing live imaging of GFP fluorescence and localization of endogenously tagged UNC-59::GFP::3xflag::AID/Septin during C. elegans development. A. Embryonic cells divisions. Embryo divisions from the 2- to 4-cell stage (time in minutes, black dashed lines depict division planes). Illustration shows dividing AB and P1 cells and UNC-59::GFP localization at cleavage furrows (yellow) B-D. Gonad morphogenesis. The distal tip cells (DTC) (white dashed line), which leads gonad outgrowth, and the rachis (yellow arrowheads) that supports the developing germline syncytium. In D, white arrowheads mark embryonic cell cleavage rings. E-G. DTC migration. The DTC throughout its L2 to young adult stage migration with septin bundles and rings present (white dashed line, yellow arrowhead indicates rachis where septin form rings in germ cells to maintain a syncytium). H-K. Sex myoblast divisions. A migrating sex myoblast (white dashed line) during the L2 stage. After migration, the sex myoblast and its descendants continue to express UNC-59 during divisions in the L3 stage once these cells form the vulval muscles (white dashed line) in the late L4 stage (ventral view, K). L-M. Pharynx, seam cells and sperm. UNC-59/Septin localization in cells of the pharynx (white dashed line, buccal cavity (bc), anterior of the procorpus (p), and terminal bulb (tb)) in epithelial seam cells, and sperm (white arrows) that have been expelled from the spermatheca (white dashed line) by an embryo during ovulation. All scale bars are 10um.
Name201220191576863937.jpg
DepictExpr_patternExpr14682
AcknowledgmentTemplateReprinted from <Journal_URL>
Publication_year2019
Article_URLDOIid10.17912/micropub.biology.000200
Journal_URLmicroPublicationBiology
Publisher_URLMicropublication
ReferenceWBPaper00058988