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WormBase Tree Display for RNAi: WBRNAi00082238

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Name Class

WBRNAi00082238HomolHomol_homolB0207:RNAi
Sequence_infoDNA_textatggagaataagccacctgtaatcaaccttccagagaaggaaactgtgaatactccacagaagggaggaaaatttactattaacgatttcgagatcggaagaccactgggaaaaggaaaattcggcagtgtttacctggctcgcacaaaaactggacatttccatgtagcgatcaaggttctattcaaatcgcaactcatctcaggaggcgtcgagcatcaattggagcgtgaaattgagattcaatctcatttgaatcacccgaatattatcaagctctacacatatttttgggatgcgaagaagatctatctcgttttggaatatgcccccggaggcgaaatgtacaagcaattgacagtgagcaagagattctcagagccaacagctgcaaaatatatgtatgagatcgcagatgctttgtcatattgccacaggaaaaatgtgattcatcgcgacattaaaccggagaatttgcttatcggatcacaaggagaattgaaaattggagattttggatggagtgttcatgctccttcgaataagcgccaaacaatgtgcggaacaatggactatctccctcctgaaatggtgaatggagccgatcacagtgacgcagttgacttatgggcaatcggcgttctctgctatgagtttctcgttggaaaacccccattcgagcacgaagatcagtccaagacatatgcggcaatcaaagcggcacggtttacttaccctgattctgtgaagaagggtgcacgagatttgattggaagattgcttgtcgttgatcccaaggctcgctgtacgcttgaacaggtgaaggaacactactggatccagggaatgatggaggcaaaaattagagctgagaagcagcaaaagattgaaaaagaagcaagtcttcggaatcactga
ExperimentLaboratoryJS
Date01 Apr 2005 00:00:00
TreatmentEmbryos were fixed and stained with DAPI and antibodies specific for alpha-tubulin and TLK-1P634 (Phosphorylated TLK-1S634).
Delivered_byBacterial_feeding
InhibitsPredicted_geneB0207.4Inferred_automaticallyRNAi_primary
GeneWBGene00000099Inferred_automaticallyRNAi_primary
TranscriptB0207.4.1Inferred_automaticallyRNAi_primary
B0207.4.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00026599
PhenotypeWBPhenotype:0001351RemarkImmunostaining of wild-type with the TLK-1P634 antibody revealed nuclear/chromatin-associated staining from early prophase through metaphase and staining of mitotic centrosomes. In air-2(RNAi) embryos the nuclear/chromatin-associated staining was strongly reduced. Because TLK-1 expression (unphosphorylated) is not dependent on AIR-2, this reveals a defect in phosphorylation. The mitotic centrosome staining was not affected.
MethodRNAi