WormBase Tree Display for RNAi: WBRNAi00095782

WBRNAi00095782 Homol: Homol_homol: Y41C4A:RNAi
  Sequence_info: PCR_product: sjj_Y41C4A.3
  Experiment: Laboratory: GR
    Date: 23 Feb 2012 00:00:00
    Treatment: Synchronized L1 larvae (approximately 60 animals in 5-15 microliter liquid volume) were serially aliquoted to the center of each bacterial lawn using a repeat pipettor. Aversion assays were typically scored at 48-58 hours (on bench top at 23 to 24 degrees Celsius), although occasionally animals were incubated with RNAi for up to 64 hours before robust aversion. Control RNAi plates become unreliable >56-58 hours due to depletion of bacteria and loss of distinct lawn boundary caused by animal locomotion. Animals were scored for aversion using a Nikon SMZ800 microscope. The number of animals located off the RNAi lawn (Noff) and the total number of animals per well (Ntotal) were counted. For the RNAi screen, wells were visually inspected prior to counting, and only samples appearing to be aversion candidates were scored. Aversion = Noff/Ntotal and was averaged across three replicates. Positives were confirmed by independent replicates (usually > 3) and sequence-verification of the RNAi clone. Approximate developmental stage was recorded for all RNAi clones tested.
    Temperature: 23
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: Y41C4A.4g Inferred_automatically: RNAi_primary
    Gene: WBGene00000793 Inferred_automatically: RNAi_primary
    Transcript: Y41C4A.4g.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00040984
  Phenotype: WBPhenotype:0000402
  Remark: (Table S1) Y41C4A.4 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
  Method: RNAi