WormBase Tree Display for RNAi: WBRNAi00096274
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WBRNAi00096274 | Homol | Homol_homol | Y105E8A:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_Y105E8C.e | |||
Experiment | Laboratory | GR | |||
Date | 23 Feb 2012 00:00:00 | ||||
Genotype | agIs26[myo-2::mCherry,clec-60::gfp] | ||||
Treatment | For reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | Y105E8A.16 | Inferred_automatically | RNAi_primary | |
Gene | WBGene00004489 | Inferred_automatically | RNAi_primary | ||
Transcript | Y105E8A.16.1 | Inferred_automatically | RNAi_primary | ||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00040984 | ||||
Phenotype | WBPhenotype:0001236 | Remark | RNAi results in moderate activation of the clec-60::GFP reporter gene | ||
Remark | (Table S2A) rps-20 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation. | ||||
Method | RNAi |