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WormBase Tree Display for Strain: WBStrain00004512

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Name Class

WBStrain00004512StatusLive
Genotypefem-3(e2006) IV.
Public_nameCB3844
ContainsGeneWBGene00001413
VariationWBVar00144475
PropertiesMutagenEMS
CGC_received16 Dec 1994 00:00:00
PhenotypeWBPhenotype:0001171Paper_evidenceWBPaper00057129
Curator_confirmedWBPerson712
RemarkWe confirmed that C. elegans grown on wildtype V. vulnificus reduced the median lifespan of animals from 12 days to 9 days. However, animals grown on the tonB1 tonB2 strain also had a median lifespan of 9 days and there was no statistically significant increase in survival of worms grown on the mutant strain (Fig. 1).Paper_evidenceWBPaper00057129
Curator_confirmedWBPerson712
ImageWBPicture0000014915Paper_evidenceWBPaper00057129
Curator_confirmedWBPerson712
Phenotype_assayTreatmentOvernight broth cultures of each V. vulnificus strain were normalized to an OD600 of 0.3, spread onto CM9 plates, and incubated for 24 hours at 35C. OP50 experiments were done on standard MYOB plates. For each replicate, 10 L4 hermaphrodites were transferred onto each plate on day 0. fem-3 animals at the restrictive temperature of 25C, which have been shown to have normal lifespan (Kenyon et al. 1993), were used so animals did not have to be transferred away from progeny. Plates incubated at 25C were scored every 24hr for the number of remaining live worms. A worm was considered dead when it no longer responded to touch with a pick.Paper_evidenceWBPaper00057129
Curator_confirmedWBPerson712
Temperature25Paper_evidenceWBPaper00057129
Curator_confirmedWBPerson712
LocationCGC
CB
Made_byWBPerson261
RemarkTemperature sensitive Fem. Hermaphrodites at 15C, female at 25C. Maintain at 15C.Inferred_automaticallyFrom CGC strain data
ReferenceWBPaper00000922
WBPaper00057129
WBPaper00065793
SpeciesCaenorhabditis elegans