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WormBase Tree Display for Strain: WBStrain00034031

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Name Class

WBStrain00034031StatusLive
Genotypeunc-62(e644) V; gaIs286.
Public_nameSD1887
ContainsGeneWBGene00006796
VariationWBVar00143365
TransgeneWBTransgene00016394
PropertiesOutcrossedx0
CGC_received12 Dec 2012 00:00:00
LocationCGC
Made_byWBPerson10375
RemarkgaIs286 [unc-62(7b)::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. A STOP-codon was inserted into exon 7a of unc-62 to generate an UNC-62(7b)-specific reporter. Recombineered fosmid was integrated by biolistic bombardment to produce strain OP602, which wa outcrossed to produce SD1894. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (URL: www.modencode.org) Derived from parental strains BC1282 and SD1894.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans