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WormBase Tree Display for Strain: WBStrain00037362

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Name Class

WBStrain00037362StatusLive
Genotypesur-6(ok3215) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Public_nameVC2495
ContainsGeneWBGene00000254
WBGene00006352
VariationWBVar00143617
WBVar00241187
WBVar00094281
RearrangementhT2
hT2[bli-4(e937) let-?(q782) qIs48]
TransgeneWBTransgene00001901
PropertiesOutcrossedx1
MutagenEMS
CGC_received25 Mar 2009 00:00:00
LocationCGC
Made_byWBPerson20180
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F26E4.1. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3215 homozygotes (sterile adult). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TCAGCCGATGTGATCTCTTG. External right primer: TGTTCAAATCCCACACCTGA. Internal left primer: GAATATTTGGCAACGGGAGA. Internal right primer: CCTTGACAGTAAGATAGTCCCTCG. Internal WT amplicon: 1263 bp. Deletion size: 438 bp. Deletion left flank: AATCAAGTATAACATTAATGATTAAATATT. Deletion right flank: GTTAACCTTTGGAATCTGGAGATTACCAAT.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans