WormBase Tree Display for Strain: WBStrain00049003
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| WBStrain00049003 | Status | Live | |
|---|---|---|---|
| Genotype | unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V. | ||
| Public_name | DLW14 | ||
| Contains | Gene | WBGene00003514 | |
| WBGene00003515 | |||
| WBGene00006745 | |||
| WBGene00006843 | |||
| Transgene | WBTransgene00009915 | ||
| Properties | Outcrossed | x3 | |
| Mutagen | CRISPR_Cas9 | ||
| CGC_received | 16 Jun 2021 00:00:00 | ||
| Location | DLW | ||
| CGC | |||
| Remark | Generated from afp_otherstrain Paper data WBPaper000612001. | ||
| krIs14 [hsp-16.48p::MosTransposase + lin-15(+) + unc-122p::GFP] V. Recessive Unc. unc-5(lib1) is a CRISPR/Cas9 engineered mutant carrying the Intersister/Intrachromatid Repair Assay (ICR Assay) cassette inserted into the endogenous unc-5 locus. Briefly, ICR assay cassette includes two tandem GFP cassettes: the upstream using the myo-3 (body wall) promoter with a truncated GFP coding sequence, and the down-stream using the myo-2 (pharynx) promoter with GFP coding sequence interrupted by a Mos1 Drosophila transposon. Excision of Mos1 yields a single DSB, which if repaired by intersister or intrachromatid recombination, then will yield GFP+ progeny. The krIs14 insertion carrying heat-shock inducible Mos1 transposase is marked with coelomocyte GFP expression. Reference: Toraason E, et al. Current Biology 2021. https: | |||
| Made_by: Marissa Glover | CGC_data_submission | ||
| Reference | WBPaper00061200 | ||
| Species | Caenorhabditis elegans |
