WormBase Tree Display for Strain: WBStrain00050744
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| WBStrain00050744 | Status | Live | ||
|---|---|---|---|---|
| Genotype | nDf67 mir-52(n4100) IV/nT1 [qIs51] (IV;V); nDf58 X, lucIs24. | |||
| Public_name | MLC903 | |||
| Contains | Gene | WBGene00003280 | ||
| Variation | WBVar00090790 | |||
| Rearrangement | nDf58 | |||
| nDf67 | ||||
| nT1 | ||||
| Transgene | WBTransgene00001903 | |||
| WBTransgene00032970 | ||||
| Properties | Outcrossed | x0 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 21 Oct 2020 00:00:00 | |||
| Location | CGC | |||
| Remark | lucIs24 [mir-52p::mirtron-51 + elt-2::dsRed + myo-2::mCherry]. Pick GFP+ animals to maintain balanced line. Balanced mir-51 family mutant expressing a mirtron-version of mir-51. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP, arrested nT1[qIs51] aneuploids, and non-GFP mir-51 family homozygous mutants. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Non-GFP mir-51 family homozygous mutants rescued by mirtron-51 transgene are viable, but slow-growing and sick. Strain is derived from injection into parental strain MT17143. lucIs24 is a spontaneous integrant originating from a complex extra-chromosomal array, the genomic location of the transgene is unknown. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press. | Inferred_automatically | From CGC strain data | |
| Made_by: Philipp Dexheimer | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
