WormBase Tree Display for Transgene: WBTransgene00000546
expand all nodes | collapse all nodes | view schema
WBTransgene00000546 | Public_name | geIn3 | |||||||
---|---|---|---|---|---|---|---|---|---|
Summary | [sir-2.1; rol-6] | ||||||||
Construction | Construct | WBCnstr00000544 | |||||||
Coinjection_other | rol-6 | ||||||||
Integration_method | Gamma_irradiation | ||||||||
Construction_summary | sir-2.1 refers to sequence R11A8.4. | ||||||||
A 2.2 kb genomic fragment of sir-2.1 was injected into N2 along with the rol-6 transformation marker. | |||||||||
Genetic_information | Integrated | ||||||||
Phenotype | WBPhenotype:0000134 | Paper_evidence | WBPaper00036476 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Overexpression of SIR-2.1 results in decreased transcription of fat-7 and lbp-6 under both fed and fasted conditions, compared to control animals. | Paper_evidence | WBPaper00036476 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Caused_by_gene | WBGene00004800 | Paper_evidence | WBPaper00036476 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001183 | Paper_evidence | WBPaper00036476 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Increased SIR-2.1 activity in nematodes results in lower levels of stored fat, as measured by Sudan Black staining. | Paper_evidence | WBPaper00036476 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Caused_by_gene | WBGene00004800 | Paper_evidence | WBPaper00036476 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005772 | PATO:0000460 | Paper_evidence | WBPaper00036476 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000061 | Paper_evidence | WBPaper00040229 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Strain (LG100) carrying the integrated transgene array geIn3 [sir-2.1 rol-6(su1006)] (ref. 1) was long-lived; however, outcrossing (x5) of geIn3 to wild type (N2) abrogated the increase in longevity. | Paper_evidence | WBPaper00040229 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Caused_by_gene | WBGene00004800 | Paper_evidence | WBPaper00040229 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Associated_with | Strain | WBStrain00007690 | |||||||
WBStrain00007691 | |||||||||
WBStrain00007692 | |||||||||
WBStrain00024177 | |||||||||
Reference (25) | |||||||||
Species | Caenorhabditis elegans |