WormBase Tree Display for Transgene: WBTransgene00005741

WBTransgene00005741 Public_name: WBPaper00032271Ex1
  Summary: [unc-18::unc-18cDNA(D214N)]
  Construction: Construct: WBCnstr00005614
  Genetic_information: Extrachromosomal
    Phenotype: WBPhenotype:0001481 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: Thrashing frequency increased Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001612 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: Transgenic animals with the D214N UNC-18 mutation completely lost any ethanol-induced hyperactivity Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: 0-100mM EtOH Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
          Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001613 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: The ability of ethanol to depress activity was significantly decreased for transgenic worms expressing the UNC-18 D214N mutation Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: 100-500mM of EtOH Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
          Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0004023 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: D214N mutants moved slightly faster (20%) than the wild-type Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000039 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: No obvious differences in lifespan Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000520 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: Animals appeared normal Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000634 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: No obvious differences in pharyngeal pumping Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000640 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: No obvious differences in egg laying Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000676 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: No obvious differences in growth rate Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000680 Paper_evidence: WBPaper00032271
        Curator_confirmed: WBPerson2021
        Remark: No differences in cholinergic release from motorneurons between wild-type and D214N mutant rescues Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Caused_by_gene: WBGene00006757 Paper_evidence: WBPaper00032271
            Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00003650 Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Paralysis assay at 1 mM aldicarb Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
          Genotype: unc-18(e81) Paper_evidence: WBPaper00032271
              Curator_confirmed: WBPerson2021
  Reference: WBPaper00032271
  Species: Caenorhabditis elegans