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WormBase Tree Display for Transgene: WBTransgene00008983

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Name Class

WBTransgene00008983Public_namecgIs55
Summary[Pric-19::PrP; Pric-19::GFP]
ConstructionConstructWBCnstr00008674
Integration_methodUV
Construction_summaryThe coding sequence for mouse PrP(1-254) carrying a human sequence-based 3F4 epitope was PCR amplified from the PrP1-254-mPrP1,28 using the following primers: 5'-GCG CGG CTA GCA TGG CGA ACC TTG GCT ACT GG-3' (forward), 5'-GCG CGG AGC TCT CAT CCC ACG ATC AGG AAG ATG A-3' (reverse). The resulting PCR products were digested with NheI/SacI and ligated to a plasmid that had been predigested with NheI/SacI: pRB490, containing the dopamine neuronal specific promoter Pdat-1,29 to create Pdat-1::PrP(1-254).
Genetic_informationIntegrated
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkLow-level pan-neuronal expression of PrP had a life span compatible to that of GFP only transgenic and non-transgenic animals.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
WBPhenotype:0000072Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkLow-level pan-neuronal expression of PrP showed normal body morphology.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
WBPhenotype:0000518Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkLow-level pan-neuronal expression of PrP did not cause any detectable harm to worms.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
WBPhenotype:0000676Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkLow level pan-neuronal expression of PrP did not cause any detectable harm to worms.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
WBPhenotype:0000904Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkAnimals showed normal phalloidin staining of F-actin suggesting normal, organized muscle architecture.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
WBPhenotype:0001641Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkAnimals showed pBoc rates comparable to non transgenic and GFP only animals.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
WBPhenotype:0001739Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
RemarkLow-level pan-neuronal expression of PrP showed comparable thrashing rates to non transgenic animals during adult stages.Paper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
Caused_by_otherMouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion proteinPaper_evidenceWBPaper00037809
Curator_confirmedWBPerson712
ReferenceWBPaper00037809
SpeciesCaenorhabditis elegans