WormBase Tree Display for Transgene: WBTransgene00008983
expand all nodes | collapse all nodes | view schema
WBTransgene00008983 | Public_name | cgIs55 | |||||
---|---|---|---|---|---|---|---|
Summary | [Pric-19::PrP; Pric-19::GFP] | ||||||
Construction | Construct | WBCnstr00008674 | |||||
Integration_method | UV | ||||||
Construction_summary | The coding sequence for mouse PrP(1-254) carrying a human sequence-based 3F4 epitope was PCR amplified from the PrP1-254-mPrP1,28 using the following primers: 5'-GCG CGG CTA GCA TGG CGA ACC TTG GCT ACT GG-3' (forward), 5'-GCG CGG AGC TCT CAT CCC ACG ATC AGG AAG ATG A-3' (reverse). The resulting PCR products were digested with NheI/SacI and ligated to a plasmid that had been predigested with NheI/SacI: pRB490, containing the dopamine neuronal specific promoter Pdat-1,29 to create Pdat-1::PrP(1-254). | ||||||
Genetic_information | Integrated | ||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Low-level pan-neuronal expression of PrP had a life span compatible to that of GFP only transgenic and non-transgenic animals. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000072 | Paper_evidence | WBPaper00037809 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Low-level pan-neuronal expression of PrP showed normal body morphology. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000518 | Paper_evidence | WBPaper00037809 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Low-level pan-neuronal expression of PrP did not cause any detectable harm to worms. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000676 | Paper_evidence | WBPaper00037809 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Low level pan-neuronal expression of PrP did not cause any detectable harm to worms. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000904 | Paper_evidence | WBPaper00037809 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Animals showed normal phalloidin staining of F-actin suggesting normal, organized muscle architecture. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001641 | Paper_evidence | WBPaper00037809 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Animals showed pBoc rates comparable to non transgenic and GFP only animals. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001739 | Paper_evidence | WBPaper00037809 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Low-level pan-neuronal expression of PrP showed comparable thrashing rates to non transgenic animals during adult stages. | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | Mouse glycosylphosphatidylinositol (GPI)-anchored glycoprotein prion protein | Paper_evidence | WBPaper00037809 | ||||
Curator_confirmed | WBPerson712 | ||||||
Reference | WBPaper00037809 | ||||||
Species | Caenorhabditis elegans |