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WormBase Tree Display for Transgene: WBTransgene00009211

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Name Class

WBTransgene00009211Public_namermIs214
Summary[pF25B3.3::257cAT3v1-1q75::yfp]
Synonym275cAT3q75
ConstructionConstructWBCnstr00008897
Integration_methodGamma_irradiation
Construction_summaryATXN3 C-terminal YFP-tagged expression constructs (lacking 257aa from the amino terminal) were generated by PCR (using full-length constructs as a template) and cloned into the BamH I sites of PF25B3.3Q0::YFP, forming PF25B3.3::257cAT3q(n)::YFP.
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000306Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
RemarkThis ATXN3 C-terminal YFP-tagged expression construct containing a 75 polyQ tract (275cAT3q75) produces a heterogenous expression pattern in expressing neurons. FRAP analysis demonstrated differences in solubility of the protein among different neurons.Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
Caused_by_otherATXN3 lacking amino terminal 257 aa, with polyQ(75) expansionPaper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
WBPhenotype:0001405Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
RemarkUnlike in animals expressing full-length AT3q14 (ataxin with polyQ(14) expansion) or YFP-alone, discrete foci formed. Unlike animals expressing AT3q75, protein aggregation was age-dependent.Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
Caused_by_otherATXN3 lacking amino terminal 257 aa, with polyQ(75) expansionPaper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
WBPhenotype:0001472Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
RemarkAnimals showed a significant reduction in the chemotaxis index when compared to N2 and control animals.Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
Caused_by_otherATXN3 C-terminal truncation with poly-Q(130) expansionPaper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0001015Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
RemarkAnimals exhibited similar rates of development to adulthood as controls.Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
Caused_by_otherATXN3 lacking amino terminal 257 aa, with polyQ(75) expansionPaper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
WBPhenotype:0001206Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
RemarkAnimals were similar to controls.Paper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
Caused_by_otherATXN3 with poly-Q(14) expansionPaper_evidenceWBPaper00038396
Curator_confirmedWBPerson712
ReferenceWBPaper00038396
SpeciesCaenorhabditis elegans