WormBase Tree Display for Transgene: WBTransgene00025323
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WBTransgene00025323 | Public_name | dahEx5 | ||||||
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Summary | [Podr-10::VPS-4 DN(E219Q); Pttx-3::dsRed] | |||||||
Construction | Construct | WBCnstr00040059 | ||||||
Coinjection_other | Pttx-3::dsRed | |||||||
Construction_summary | Mutation of Glutamate 219 to Glutamine (E219Q) in VPS-4 results in a dominant negative enzyme (VPS-4 DN). | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000147 | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Expression of VPS-4 DN had no effect on the ability of animals to react to the chemoattractant diacetyl under well-fed conditions (Figure 1C). However, VPS-4 DN expression in the AWA neuron resulted in significantly lower chemotaxis indices, compared to wild type, fed animals (Figure 1C, asterisk). | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson712 | |||||||
Image | WBPicture0000013881 | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | Starvation | Paper_evidence | WBPaper00054955 | ||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | odr-10p::gfp | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000306 | Paper_evidence | WBPaper00054955 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | VPS-4 DN increased the frequency with which we observed animals with aberrant ODR-10 accumulation (Figure 1B). In 8 animals (out of 58) there was observable fluorescence in the cell bodies of AWA neurons (1 with fluorescence exclusively in the cell body). Starvation exacerbated this effect: 26 out of 39 animals had fluorescence in the cell bodies of AWA neurons (7 with fluorescence exclusively in the cell body). We also observed that starvation of animals led toincreased frequency of visible fluorescence in the cilia of AWA in WT animals (Figure 1B). | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson712 | |||||||
Image | WBPicture0000013881 | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Genotype | odr-10p::gfp | Paper_evidence | WBPaper00054955 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0002038 | Paper_evidence | WBPaper00054955 | ||||||
Curator_confirmed | WBPerson10038 | |||||||
Caused_by_other | VPS-4 dominant negative | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson10038 | |||||||
Phenotype_assay | Genotype | kyIs53 | Paper_evidence | WBPaper00054955 | ||||
Curator_confirmed | WBPerson10038 | |||||||
Phenotype_not_observed | WBPhenotype:0000303 | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson10038 | |||||||
Caused_by_other | VPS-4 dominant negative | Paper_evidence | WBPaper00054955 | |||||
Curator_confirmed | WBPerson10038 | |||||||
Phenotype_assay | Genotype | kyIs53 | Paper_evidence | WBPaper00054955 | ||||
Curator_confirmed | WBPerson10038 | |||||||
Associated_with | Strain | WBStrain00042518 | ||||||
Reference | WBPaper00054955 | |||||||
Species | Caenorhabditis elegans |