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WormBase Tree Display for Transgene: WBTransgene00025323

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Name Class

WBTransgene00025323Public_namedahEx5
Summary[Podr-10::VPS-4 DN(E219Q); Pttx-3::dsRed]
ConstructionConstructWBCnstr00040059
Coinjection_otherPttx-3::dsRed
Construction_summaryMutation of Glutamate 219 to Glutamine (E219Q) in VPS-4 results in a dominant negative enzyme (VPS-4 DN).
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000147Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
RemarkExpression of VPS-4 DN had no effect on the ability of animals to react to the chemoattractant diacetyl under well-fed conditions (Figure 1C). However, VPS-4 DN expression in the AWA neuron resulted in significantly lower chemotaxis indices, compared to wild type, fed animals (Figure 1C, asterisk).Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
ImageWBPicture0000013881Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
Phenotype_assayTreatmentStarvationPaper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
Genotypeodr-10p::gfpPaper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
WBPhenotype:0000306Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
RemarkVPS-4 DN increased the frequency with which we observed animals with aberrant ODR-10 accumulation (Figure 1B). In 8 animals (out of 58) there was observable fluorescence in the cell bodies of AWA neurons (1 with fluorescence exclusively in the cell body). Starvation exacerbated this effect: 26 out of 39 animals had fluorescence in the cell bodies of AWA neurons (7 with fluorescence exclusively in the cell body). We also observed that starvation of animals led toincreased frequency of visible fluorescence in the cilia of AWA in WT animals (Figure 1B).Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
ImageWBPicture0000013881Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
Phenotype_assayGenotypeodr-10p::gfpPaper_evidenceWBPaper00054955
Curator_confirmedWBPerson712
WBPhenotype:0002038Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson10038
Caused_by_otherVPS-4 dominant negativePaper_evidenceWBPaper00054955
Curator_confirmedWBPerson10038
Phenotype_assayGenotypekyIs53Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson10038
Phenotype_not_observedWBPhenotype:0000303Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson10038
Caused_by_otherVPS-4 dominant negativePaper_evidenceWBPaper00054955
Curator_confirmedWBPerson10038
Phenotype_assayGenotypekyIs53Paper_evidenceWBPaper00054955
Curator_confirmedWBPerson10038
Associated_withStrainWBStrain00042518
ReferenceWBPaper00054955
SpeciesCaenorhabditis elegans