WormBase Tree Display for Variation: WBVar00000443

WBVar00000443 Evidence: Paper_evidence: WBPaper00031977
  Name: Public_name: bc248
  Sequence_details: Mapping_target: T24B8
    Type_of_mutation: Deletion
    SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Laboratory: MD
    Status: Live
  Affects: Gene: WBGene00001950
    Interactor: WBInteraction000503686
  Description: Phenotype: WBPhenotype:0000006 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Animals accumulated twice as many eggs as wild-type; however they were capable of laying eggs. Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
      WBPhenotype:0000114 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: An hlh-3-like transcript was generated consisting of an anomalous upstream exon directly linked, in-frame, to exon II, as determined by RT-PCR. Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
      WBPhenotype:0000222 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: 65% of HSN's lacked 5HT, as determined by absense of immunoreactivity to antibodies directed agains 5HT. In addition 65% of HSNs lacked expression of tph-1 reporter gene (tph-1::gfp) (n=194). Based on the same assays, serotonin metabolism was normal in the serotonergic neurosecretory motor neurons (NSMs) (data not shown). Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006830 PATO:0000460 Paper_evidence: WBPaper00031977
                Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: tph-1::gfp and or UNC-40::gfp Paper_evidence: WBPaper00031977
              Curator_confirmed: WBPerson712
      WBPhenotype:0001224 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Among detectable HSNs, approximately 70% had grossly abnormal axon projections, including axons that projected laterally or wandered aimlessly; these misdirected axons lacked obvious varicosities. As assayed by 5HT immunostaining. Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006830 PATO:0000460 Paper_evidence: WBPaper00031977
                Curator_confirmed: WBPerson712
      WBPhenotype:0001340 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Animals were insensitive to exogenous imiprimine, 66.2% of animals laid two or less eggs in the presence of imiprimine (n=150); however, 5% of individuals were sensitive and released ten or more eggs. Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
      WBPhenotype:0001410 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: At least 25% of HSNs and 75% of VCs lacked UNC-40::gfp expression. Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006830 PATO:0000460 Paper_evidence: WBPaper00031977
                Curator_confirmed: WBPerson712
            WBbt:0004432 PATO:0000460 Paper_evidence: WBPaper00031977
                Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: UNC-40::gfp Paper_evidence: WBPaper00031977
              Curator_confirmed: WBPerson712
      WBPhenotype:0002490 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Among detectable HSNs, additional axons projected posteriorly from the cell soma on rare occasions. As assayed by 5HT immunostaining. Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006830 PATO:0000460 Paper_evidence: WBPaper00031977
                Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000511 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Animals had properly localized HSN nuclei (n=30). Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006830 PATO:0000460 Paper_evidence: WBPaper00031977
                Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: unc-86::gfp Paper_evidence: WBPaper00031977
              Curator_confirmed: WBPerson712
      WBPhenotype:0000612 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Animals appeared to have a mostly functional nervous system (data not shown). Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
      WBPhenotype:0001629 Paper_evidence: WBPaper00031977
        Curator_confirmed: WBPerson712
        Remark: Animals were insensitive to the egg-laying inducing effects of 5HT (serotonin) (n=50). Paper_evidence: WBPaper00031977
            Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00031977
          Curator_confirmed: WBPerson712
  Reference: WBPaper00031977
  Method: Deletion_allele