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WormBase Tree Display for Variation: WBVar00054167

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Name Class

WBVar00054167EvidencePaper_evidenceWBPaper00042307
NamePublic_namech3
Other_nameF46C8.5.1:c.197+1_352del
HGVSgCHROMOSOME_X:g.7530116_7531392del
Sequence_detailsSMapS_parentSequenceF46C8
Flanking_sequencesagatcggttcgtttcaaaggtcaacggaagatgttgttcgaaaagcatcgaatcatgtct
Mapping_targetF46C8
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00034657
LaboratoryTB
StatusLive
AffectsGeneWBGene00000438
TranscriptF46C8.5.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScF46C8.5.1:c.197+1_352del
cDNA_position?-352
CDS_position?-352
Protein_position?-118
Intron_number2-3/10
Exon_number3-4/11
Interactor (25)
GeneticsInterpolated_map_positionX-1.62835
Mapping_dataIn_multi_point4203
DescriptionPhenotypeWBPhenotype:0000572Paper_evidenceWBPaper00042307
Curator_confirmedWBPerson1133
WBPhenotype:0001224Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Remark"To analyze axon migration, we therefore chose only animals in which unc-53:GFP intensity in the ALA cell body was comparable with wild type. Unexpectedly, ALA axons could not be identified in the vast majority of these ceh-14(ch3) unc-53:GFP+ animals (Fig 6D), indicating that CEH-14 functions at an early stage of axon projection... In the few ceh-14(ch3) animals in which we did observe lateral ALA axons, they extended well past the midbody, and the majority completed their migration to the tail (Fig 6B,D)... As CEH-14 is required for wild-type levels of CEH-17 in ALA, we would expect to see a ceh-17-like axon migration defect among the rare axons that do extend in the ceh-14 mutant; and indeed, some of the ALA axons in ceh-14(ch3) stop short of the tail (Fig 6D)."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0003955PATO:0000460Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeunc-53:GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
WBPhenotype:0001278Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Remark"We found that the ceh-14(ch3) mutation nearly abolished the expression of let-23/EGFR and severely affected the expression of plc-3/PLC-γ specifically within ALA (Table 2, Fig 1A,B)."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
The ceh-14(ch3) null mutant exhibits decreased expression of the ver-3::GFP transgene in the ALA neuron (Table 2)Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
The ceh-14(ch3) null mutant exhibits decreased expression of the ida-1::GFP transgene in the ALA neuron (Table 2, Figure 3)Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
The ceh-14(ch3) null mutant exhibits decreased expression of the flp-7::GFP transgene in the ALA neuron (Table 2, Figure 3)Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
"We then investigated whether CEH-14 regulates the expression of a ceh-17:GFP reporter (pNP69; kindly provided by N. Pujol) which is normally expressed in ALA and the four SIA neurons (Pujol et_al, 2000). We found that ALA-specific expression of ceh-17:GFP was nearly abolished in the ceh-14 mutant throughout development (Fig 2B)."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
"We found that in ceh-14(ch3) animals, ceh-14:GFP expression in ALA is compromised at all stages examined, with a fraction of animals showing no detectable expression in ALA (Fig 2C), and the remaining animals having an ALA fluorescence intensity significantly lower than wild type (not shown). This autoregulatory effect is of a magnitude that makes it unlikely to be an indirect consequence of CEH-14-CEH-17 cross-regulatory interactions, as a complete loss of CEH-17 produces relatively mild defects in ceh-14 expression."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
"We analyzed the expression of des-2:GFP in the ALA neuron of ceh-14 and ceh-17 null mutant animals and found that at the L1 stage, reporter expression is dependent on CEH-14 but not on CEH-17 (Fig 4B), consistent with our observed genetic results with deg-3(gf). At the L4 stage, however, deg-3 expression is dependent on both factors (Fig 4B,C), revealing different temporal requirements for CEH-17 and CEH-14 in deg-3 expression."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
"We then examined unc-53:GFP expression in ceh-14 null mutant animals, and observed a mild but significant decrease in reporter expression, indicating that CEH-14 affects unc-53 transcription in ALA (Fig 6A)."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0003955PATO:0000460Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Phenotype_assayGenotypelet-23:LET-23-GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
plc-3::YFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
ver-3::GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
ida-1::GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
flp-7::GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
ceh-17::GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
ceh-14::GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
des-2:GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
unc-53:GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
WBPhenotype:0001524Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Remark100% of ceh-14(ch3) animals were resistant to EGF-induced sleep (Table 1); "We found that similar to ceh-17 animals, the ALA neuron is present and its cell body is positioned normally (Fig 1A, DIC images) but the ceh-14(ch3) null mutant animals are completely resistant to the behavioral effects of EGF expression (Table 1)."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
PenetranceComplete100Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAnimals carrying the hs:LIN-3 transgene were well fed and grown at 20C. Young adult animals were scored 2 hours after heat shock for EGF-induced sleep behavior (see Materials and methods).Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Genotypehs:LIN-3Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
WBPhenotype:0002211Paper_evidenceWBPaper00042307
Curator_confirmedWBPerson1133
WBPhenotype:0002433Paper_evidenceWBPaper00050011
Curator_confirmedWBPerson712
RemarkResistance to pumping quiescence after heatshock in flp-13 mutants was much weaker than the negative controls, ceh-14 and ceh-17, suggesting that flp-13 is not the only neuropeptide necessary for pumping quiescence during stress-induced sleep (flp-13: 79% 3% pumping quiescent compared to ceh-14: 0% 0% and compared to ceh-17: 5% 3%; p < 0.001; Figure 2A). The ceh-14 and ceh-17 mutants, previously shown to bestrongly resistant to heat shock [7, 14], displayed locomotionquiescence after heat shock (ceh-14: 0% 0% locomotionquiescent before heat shock compared to ceh-14: 36% 4%locomotion quiescent after heat shock; ceh-17: 0% 0% locomotionquiescent before heat shock compared to ceh-17:56% 5% locomotion quiescent after heat shock; p < 0.01;Figure 2B).Paper_evidenceWBPaper00050011
Curator_confirmedWBPerson712
WBPhenotype:0002512Paper_evidenceWBPaper00042307
Curator_confirmedWBPerson1133
Phenotype_not_observedWBPhenotype:0000306Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
RemarkThe ceh-14(ch3) null mutant exhibits no change in expression of the unc-119::YFP transgene in the ALA neuron (Table 2)Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
The ceh-14(ch3) null mutant exhibits no change in expression of the rab-3::GFP transgene in the ALA neuron (Table 2)Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0003955PATO:0000460Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeunc-119::YFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
rab-3::GFPPaper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
WBPhenotype:0001310Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
Remark"We found that similar to ceh-17 animals, the ALA neuron is present and its cell body is positioned normally (Fig 1A, DIC images) but the ceh-14(ch3) null mutant animals are completely resistant to the behavioral effects of EGF expression (Table 1)."Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0003955PATO:0000460Paper_evidenceWBPaper00036308
Curator_confirmedWBPerson2987
ReferenceWBPaper00042307
WBPaper00036308
WBPaper00011591
WBPaper00050011
WBPaper00066004
MethodDeletion_allele