WormBase Tree Display for Variation: WBVar00089076
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WBVar00089076 | Evidence | Paper_evidence | WBPaper00000565 | ||||||
---|---|---|---|---|---|---|---|---|---|
Name | Public_name | mn160 | |||||||
Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00034251 | ||||||||
Laboratory | SP | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00011559 | |||||||
Genetics | Mapping_data | In_multi_point | 370 | ||||||
Description | Phenotype | WBPhenotype:0000032 | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals produce large numbers of sick progeny (32.1% versus 0.4% for wild type). | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000050 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibit only a slight increase in the number of inviable zygotes (1.6% ) versus wild type (0.2%). | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000064 | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | hermaphrodites less viable (< 50%) than males at 25C, equal viability at 15C. | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25 | Person_evidence | WBPerson261 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Ease_of_scoring | ES1_Very_hard_to_score | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000066 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | At 25C, over two-thirds of the cross progeny from rad-6 unc-36 X rad-6 were male, whereas at 15C, the ratio is roughly equal. All self-progeny of rad-6 unc-36 are hermaphrodites at 15C and 25C. The sex ratio is normal for cross progeny from both unc-36 X rad-6 males and rad-6 unc-36 X N2 at 25C. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25C | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000154 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 25C | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Temperature | 20C | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000275 | Paper_evidence | WBPaper00000565 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibit moderate levels of UV hypersensitivity versus extreme hypersensitivity. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
hypersensitive to UV | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | UV sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 1Jm 10X-2/sec. Irradiated animals were protected from light. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000583 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals have a slightly Dpy appearance, which is more pronounced in hermaphrodites and has variable expressivity. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000742 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals show slight but statistically significant deviations from the wild-type recombination frequency between the dpy-7 and unc-3 interval. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Recombination frequencies were measured using unlinked intervals, dpy-10 unc-4 or dpy-7 unc-3. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000784 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Male fertility was 34% that of wild type. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001666 | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | hypersensitive to X irradiation | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000228 | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Non-Unc revertants of unc-58 were not observed from among 24 plates screened. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Genotype | unc-58(e665) | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000276 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | X-ray sensitivities were measured as % survival based on radiation dose. Alkaline bleach isolated eggs ranging in age from 30-180 minutes were aliquoted to plates to give between 25-300 survivors/plate. The plated eggs were irradiated at different doses at a rate of 470 r/min. Survival was scored as the % animals reaching adulthood 4-6 days after irradiation relative to un-irradiated controls. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000392 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000498 | Paper_evidence | WBPaper00000565 | |||||||
Person_evidence | WBPerson261 | ||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | not hypersensitive to MMS | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Recessive | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were tested in two chronic and 1 acute drug exposure assays: 1. gravid adults were placed on plates containing various concentrations of MMS and allowed to develop; 2. L4 larvae were incubated on plates containing various concentrations of MMS for 24 hours, their progeny were scored for predominant stage of developmental arrest after further incubation; 3. acute exposure was assayed by incubating eggs in 50mM MMS for various times before plating and ounting egg hatch and survival to adulthood. | Paper_evidence | WBPaper00000565 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001175 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001631 | Paper_evidence | WBPaper00000565 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Males produced equal numbers of X-bearing and nullo-X-bearing sperm. | Paper_evidence | WBPaper00000565 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00000565 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Disease_info | Models_disease | DOID:0050833 | |||||||
Models_disease_in_annotation | WBDOannot00000411 | ||||||||
Reference | WBPaper00000565 | ||||||||
Method | Allele |