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WormBase Tree Display for Variation: WBVar00091546

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Name Class

WBVar00091546NamePublic_nameok243
Sequence_detailsMapping_targetD2030
PCR_productOK243_external
OK243_internal
SeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00031343
WBStrain00055595
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00008415
GeneticsMapping_dataIn_multi_point5298
DescriptionPhenotypeWBPhenotype:0000031Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Remark"When fed E. coli OP50, all four mutants exhibited slow growth compared to wild-type animals (Figure 5C)."Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
WBPhenotype:0000136Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
RemarkOn a Comamonas DA1877 diet, mce-1(ok243) animals exhibited increased mRNA levels of endogenous acdh-1, acdh-2, ech-6, F09F7.4, nhr-68, and cth-1Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms fed on a Comamonas DA1877 dietPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
WBPhenotype:0000137Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
RemarkOn a Comamonas DA1877 diet, mce-1(ok243) animals exhibited reduced mRNA levels of endogenous cbs-1Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms fed on a Comamonas DA1877 dietPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
WBPhenotype:0001236Paper_evidenceWBPaper00042204
WBPaper00044854
Curator_confirmedWBPerson2987
RemarkTable S1Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
"We obtained deletion mutants for two genes involved in BCAA breakdown (mce-1 and pcca-1) and one from the methionine metabolism pathway (cbl-1) (Figure 3). These deletions were introduced into the Pacdh-1::GFP dietary sensor strain. Deletions in mce-1 and pcca-1 both caused increased GFP expression on all three diets, and a deletion in cbl-1 caused increased GFP on Comamonas DA1877, but not on E_coli OP50 (Figure S5)."Paper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
"We used two deletion mutants in each of these pathways to test whether they are also involved in mediating the response to vitamin B12. Indeed, supplementation of vitamin B12 to either propionyl-CoA breakdown or methionine/SAM cycle mutants failed to repress acdh-1 promoter activity (Figure S2D)."Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Mutation resulted in a loss of repression of expression of the Pacdh-1::GFP transgene in response to a Comamonas aq. DA1877 diet (Figure S2D)Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00005477Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
WBMol:00007769Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentWorms fed on a Comamonas DA1877 dietPaper_evidenceWBPaper00042204
Curator_confirmedWBPerson2987
Animals were fed E. coli OP50 supplemented with 64 nanomolar adenosyl-cobalaminPaper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Animals were fed E. coli OP50 supplemented with 64 nanomolar methyl-cobalaminPaper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Animals were fed a Comamonas aq. DA1877 dietPaper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
GenotypePacdh-1::GFPPaper_evidenceWBPaper00042204
WBPaper00044854
Curator_confirmedWBPerson2987
WBPhenotype:0001663Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
Phenotype_not_observedWBPhenotype:0000049Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
RemarkData not shown.Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
WBPhenotype:0000207Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
RemarkData not shown.Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
WBPhenotype:0000634Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
RemarkData not shown.Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
WBPhenotype:0000673Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
RemarkData not shown.Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
WBPhenotype:0000676Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Remark"Surprisingly, supplementation of vitamin B12 accelerated development in Dmce-1 and Dpcca-1 mutant animals but failed to increase developmental rate in Dmetr-1 and Dsams-1 mutants (Figure 5C)."Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00007769Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
WBMol:00005477Paper_evidenceWBPaper00044854
Curator_confirmedWBPerson2987
WBPhenotype:0001739Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
RemarkData not shown.Paper_evidenceWBPaper00025019
Curator_confirmedWBPerson557
Disease_infoModels_diseaseDOID:14749
Models_disease_in_annotationWBDOannot00000162
ReferenceWBPaper00042204
WBPaper00025019
WBPaper00044854
RemarkLast updated on 29 Nov 2002
Generated by the C. elegans Gene Knockout Consortium
MethodKO_consortium_allele