WormBase Tree Display for Variation: WBVar00091546
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WBVar00091546 | Name | Public_name | ok243 | |||||
---|---|---|---|---|---|---|---|---|
Sequence_details | Mapping_target | D2030 | ||||||
PCR_product | OK243_external | |||||||
OK243_internal | ||||||||
SeqStatus | Pending_curation | |||||||
Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Strain | WBStrain00031343 | |||||||
WBStrain00055595 | ||||||||
Laboratory | RB | |||||||
Person | WBPerson46 | |||||||
KO_consortium_allele | ||||||||
Status | Live | |||||||
Affects | Gene | WBGene00008415 | ||||||
Genetics | Mapping_data | In_multi_point | 5298 | |||||
Description | Phenotype | WBPhenotype:0000031 | Paper_evidence | WBPaper00044854 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "When fed E. coli OP50, all four mutants exhibited slow growth compared to wild-type animals (Figure 5C)." | Paper_evidence | WBPaper00044854 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000136 | Paper_evidence | WBPaper00042204 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | On a Comamonas DA1877 diet, mce-1(ok243) animals exhibited increased mRNA levels of endogenous acdh-1, acdh-2, ech-6, F09F7.4, nhr-68, and cth-1 | Paper_evidence | WBPaper00042204 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | Worms fed on a Comamonas DA1877 diet | Paper_evidence | WBPaper00042204 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000137 | Paper_evidence | WBPaper00042204 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | On a Comamonas DA1877 diet, mce-1(ok243) animals exhibited reduced mRNA levels of endogenous cbs-1 | Paper_evidence | WBPaper00042204 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | Worms fed on a Comamonas DA1877 diet | Paper_evidence | WBPaper00042204 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001236 | Paper_evidence | WBPaper00042204 | ||||||
WBPaper00044854 | ||||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Table S1 | Paper_evidence | WBPaper00042204 | |||||
Curator_confirmed | WBPerson2987 | |||||||
"We obtained deletion mutants for two genes involved in BCAA breakdown (mce-1 and pcca-1) and one from the methionine metabolism pathway (cbl-1) (Figure 3). These deletions were introduced into the Pacdh-1::GFP dietary sensor strain. Deletions in mce-1 and pcca-1 both caused increased GFP expression on all three diets, and a deletion in cbl-1 caused increased GFP on Comamonas DA1877, but not on E_coli OP50 (Figure S5)." | Paper_evidence | WBPaper00042204 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
"We used two deletion mutants in each of these pathways to test whether they are also involved in mediating the response to vitamin B12. Indeed, supplementation of vitamin B12 to either propionyl-CoA breakdown or methionine/SAM cycle mutants failed to repress acdh-1 promoter activity (Figure S2D)." | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Mutation resulted in a loss of repression of expression of the Pacdh-1::GFP transgene in response to a Comamonas aq. DA1877 diet (Figure S2D) | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00005477 | Paper_evidence | WBPaper00044854 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBMol:00007769 | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Treatment | Worms fed on a Comamonas DA1877 diet | Paper_evidence | WBPaper00042204 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Animals were fed E. coli OP50 supplemented with 64 nanomolar adenosyl-cobalamin | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Animals were fed E. coli OP50 supplemented with 64 nanomolar methyl-cobalamin | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Animals were fed a Comamonas aq. DA1877 diet | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Genotype | Pacdh-1::GFP | Paper_evidence | WBPaper00042204 | |||||
WBPaper00044854 | ||||||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001663 | Paper_evidence | WBPaper00025019 | ||||||
Curator_confirmed | WBPerson557 | |||||||
Phenotype_not_observed | WBPhenotype:0000049 | Paper_evidence | WBPaper00025019 | |||||
Curator_confirmed | WBPerson557 | |||||||
Remark | Data not shown. | Paper_evidence | WBPaper00025019 | |||||
Curator_confirmed | WBPerson557 | |||||||
WBPhenotype:0000207 | Paper_evidence | WBPaper00025019 | ||||||
Curator_confirmed | WBPerson557 | |||||||
Remark | Data not shown. | Paper_evidence | WBPaper00025019 | |||||
Curator_confirmed | WBPerson557 | |||||||
WBPhenotype:0000634 | Paper_evidence | WBPaper00025019 | ||||||
Curator_confirmed | WBPerson557 | |||||||
Remark | Data not shown. | Paper_evidence | WBPaper00025019 | |||||
Curator_confirmed | WBPerson557 | |||||||
WBPhenotype:0000673 | Paper_evidence | WBPaper00025019 | ||||||
Curator_confirmed | WBPerson557 | |||||||
Remark | Data not shown. | Paper_evidence | WBPaper00025019 | |||||
Curator_confirmed | WBPerson557 | |||||||
WBPhenotype:0000676 | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Surprisingly, supplementation of vitamin B12 accelerated development in Dmce-1 and Dpcca-1 mutant animals but failed to increase developmental rate in Dmetr-1 and Dsams-1 mutants (Figure 5C)." | Paper_evidence | WBPaper00044854 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00007769 | Paper_evidence | WBPaper00044854 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBMol:00005477 | Paper_evidence | WBPaper00044854 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001739 | Paper_evidence | WBPaper00025019 | ||||||
Curator_confirmed | WBPerson557 | |||||||
Remark | Data not shown. | Paper_evidence | WBPaper00025019 | |||||
Curator_confirmed | WBPerson557 | |||||||
Disease_info | Models_disease | DOID:14749 | ||||||
Models_disease_in_annotation | WBDOannot00000162 | |||||||
Reference | WBPaper00042204 | |||||||
WBPaper00025019 | ||||||||
WBPaper00044854 | ||||||||
Remark | Last updated on 29 Nov 2002 | |||||||
Generated by the C. elegans Gene Knockout Consortium | ||||||||
Method | KO_consortium_allele |