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WormBase Tree Display for Variation: WBVar00091688

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Name Class

WBVar00091688NamePublic_nameok393
Other_nameF28H6.1a.1:c.724-42_1243del
F28H6.1b.1:c.724-42_1243del
HGVSgCHROMOSOME_X:g.14150221_14150939del
Sequence_detailsSMapS_parentSequenceCHROMOSOME_X
Flanking_sequencestcttacttacaattatttaaaatatttgaagggtcccagctaagcggcttggtgccggtc
Mapping_targetCHROMOSOME_X
Type_of_mutationDeletion
PCR_productOK393_external
OK393_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00035581
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00000103
TranscriptF28H6.1a.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScF28H6.1a.1:c.724-42_1243del
cDNA_position?-1250
CDS_position?-1243
Protein_position?-415
Intron_number6-8/10
Exon_number7-9/11
F28H6.1b.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScF28H6.1b.1:c.724-42_1243del
cDNA_position?-1250
CDS_position?-1243
Protein_position?-415
Intron_number6-8/9
Exon_number7-9/10
Interactor (12)
IsolationMutagenUV/TMP
GeneticsMapping_dataIn_multi_point4152
DescriptionPhenotypeWBPhenotype:0000061Paper_evidenceWBPaper00036474
WBPaper00045812
Curator_confirmedWBPerson2987
Remark"Both akt-1(ok525) and akt-2(ok393) mutants showed a reproducible increase in lifespan relative to wild type (Fig 3g and Supplementary Table 1)."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
"Mutation in akt-2 exhibited greater lifespan extension of daf-16(mgDf50); daf-16a transgenic animals than a mutation in akt-1 (Fig 3h and Supplementary Table 1)."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
"... consistent with the DAF-16 nuclear translocation data, mutation in akt-1 resulted in a larger increase in lifespan of daf-16(mgDf50); daf-16d/f transgenic animals when compared with a mutation in akt-2 (Fig 3i and Supplementary Table 1)."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Table S1Paper_evidenceWBPaper00045812
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-16(mgDf50); DAF-16a::GFPPaper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
daf-16(mgDf50); DAF-16d/f::GFPPaper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
WBPhenotype:0000731Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Remarkakt-2 loss-of-function mutants exhibited increased sensitivity to DNA-damage-induced germ-cell apoptosis 12, 24, and 36 hr after irradiationPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006796PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentTreated with 60 Gy IRPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001781Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkENU caused a significant increase in germline apoptosis in akt-1 and akt-2 loss of- function mutantsPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006796PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentTreated with 5mM ENUPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001999Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkThe integration of signals for attraction to diacetyl (100x dilute) and avoidance from copper (100 millimolar) was impaired in akt-2(ok393) insulin-like signaling pathway mutants, resulting in more animals crossing the copper barrier to get to the diacetyl spot than in wild type controls (Figure 1C)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBMol:00002819Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000308Paper_evidenceWBPaper00036472
Curator_confirmedWBPerson2021
Remarkakt-2 mutants did not undergo significant dauer arrest at 25C or 27C on standard NGM plates containing cholesterol, similar to wildtypePaper_evidenceWBPaper00036472
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00036472
Curator_confirmedWBPerson2021
WBPhenotype:0000436Paper_evidenceWBPaper00028886
Curator_confirmedWBPerson48
RemarkLocalization of the synaptic protein SNB-1 is normal, based on transgene expression analysis.Paper_evidenceWBPaper00028886
Curator_confirmedWBPerson48
WBPhenotype:0000481Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals did not display an abnormal aversion response to copper, compared to wild type animals (Figure 2B)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0000730Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkNone of the akt mutants affected developmental apoptosisPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0000885Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkNone of the akt mutants affected the engulfment rates of the germ-cell corpsesPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001470Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutants exhibited no change in chemotaxis towards diacetyl, compared to wild type controls (Figure 2A)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002819Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0004023Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals exhibit a wild type frequency of body bends (Figure 3A)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
ReferenceWBPaper00037970
WBPaper00028886
WBPaper00029085
WBPaper00036472
WBPaper00036474
WBPaper00045812
RemarkLast updated on 29 Nov 2002
Knockout originally requested for F28H6.1 before it was renamed to F28H6.1a
Sequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele