Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00094648

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00094648NamePublic_nameor10
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00007263
WBStrain00007265
LaboratoryEU
StatusLive
AffectsGeneWBGene00003394
InteractorWBInteraction000500006
WBInteraction000500007
WBInteraction000500008
WBInteraction000500009
WBInteraction000500016
DescriptionPhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RemarkData not shownPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
PenetranceHighPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0000038Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RemarkData not shownPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0000052Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
PenetranceComplete100 percentPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
Maternal
WBPhenotype:0000643Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RemarkData not shownPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0000697Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RemarkData not shownPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0000760Paper_evidenceWBPaper00002870
WBPaper00003645
Curator_confirmedWBPerson2021
WBPerson2987
RemarkABar divides roughly parallel (instead of orthogonal) to the other AB descendants in mom mutantsPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
In mom-1(or10) mutants, the EMS cell exhibited spindle orientation defects, with significant dorsal/ventral or left/right orientation components, in contrast to the normally anterior/posterior orientation of the wild type EMS spindle (data not shown).Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
In EMS cells derived from isolated P1 blastomeres from mom-1(or10) mutants, spindles exhibited orientation defects, with a wide range of orientation angles, showing nearly random orientations (Figure 2).Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
In EMS blastomeres isolated from wild type embryos and placed in contact with isolated mom-1(or10) mutant P2 blastomeres, EMS spindles exhibited orientation defects suggesting that mom-1 is required in P2 for proper EMS spindle orientation (Figure 4A).Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
PenetranceComplete100 percentPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006411PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBbt:0006876PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
WBbt:0006873PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00002870
WBPaper00003645
Curator_confirmedWBPerson2021
WBPerson2987
WBls:0000008PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
WBls:0000071PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
Maternal
Phenotype_assayTreatmentAuthors isolated the smaller, posterior-most blastomere in a two-cell-stage embryo, called P1, and allowed it to divide into its daughters, P2 and EMS. The orientation of the mitotic spindle in EMS was then measured relative to the plane of contact between EMS and P2.Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
For genetically mosaic partial embryos, wild-type and mutant early EMS and P2 blastomeres were concurrently isolated. EMS and P2 were recombined early in the EMS cell cycle allowing the cells to be in contact for at least 9 min before EMS cytokinesis.Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
WBPhenotype:0001635Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RemarkMutant embryos make large amounts of pharynxPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
Maternal
Phenotype_assayTreatmentstaining with 9.2.1 antibody (stains pharyngeal-specific muscle cells)Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
Temperature20CPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0001637Paper_evidenceWBPaper00002870
WBPaper00005375
Curator_confirmedWBPerson2021
WBPerson2987
RemarkMost mutant embryos entirely lack intestinal cellsPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
48% of mom-1(or10) embryos lacked an intestine (Table 1)Paper_evidenceWBPaper00005375
Curator_confirmedWBPerson2987
PenetranceIncomplete48Paper_evidenceWBPaper00005375
Curator_confirmedWBPerson2987
High85 percentPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0005772PATO:0000460Paper_evidenceWBPaper00005375
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
Maternal
Phenotype_assayTreatmentIntact embryos were examined by scoring intestinal birefringencePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
Temperature20CPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0001968Paper_evidenceWBPaper00005116
Curator_confirmedWBPerson2987
RemarkTable 4Paper_evidenceWBPaper00005116
Curator_confirmedWBPerson2987
WBPhenotype:0002216Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
RemarkThe mom-1(or10) mutation resulted in loss of POP-1 asymmetric expression in MS/E and ABxxa/p pairs of daughter cells in 8-cell stage and 12-cell stage embryos (Table 1).Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004458PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0004804PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006707PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0005865PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006162PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006653PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006341PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006279PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006581PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBbt:0006451PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
Life_stageWBls:0000072PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
WBls:0000071PATO:0000460Paper_evidenceWBPaper00003574
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000760Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
RemarkIn EMS blastomeres isolated from mom-1(or10) mutant embryos and placed in contact with isolated wild type P2 blastomeres, EMS spindles exhibited wild type orientation suggesting that mom-1 is dispensable in EMS for proper EMS spindle orientation (Figure 4A).Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006876PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
WBbt:0006873PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentFor genetically mosaic partial embryos, wild-type and mutant early EMS and P2 blastomeres were concurrently isolated. EMS and P2 were recombined early in the EMS cell cycle allowing the cells to be in contact for at least 9 min before EMS cytokinesis.Paper_evidenceWBPaper00003645
Curator_confirmedWBPerson2987
WBPhenotype:0001133Paper_evidenceWBPaper00005375
Curator_confirmedWBPerson2987
Remarkmom-1(or10) animals exhibited no embryos with aberrant L-R division of EMS (Table 1)Paper_evidenceWBPaper00005375
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006876PATO:0000460Paper_evidenceWBPaper00005375
Curator_confirmedWBPerson2987
WBPhenotype:0001302Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RemarkP granule localization is normalPaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentstaining with OIC1D4 monoclonal antibody (stains P granules)Paper_evidenceWBPaper00002870
Curator_confirmedWBPerson2021
WBPhenotype:0001962Paper_evidenceWBPaper00006440
Curator_confirmedWBPerson2987
RemarkTable 4Paper_evidenceWBPaper00006440
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006863PATO:0000460Paper_evidenceWBPaper00006440
Curator_confirmedWBPerson2987
GO_termGO:0008406PATO:0000460Paper_evidenceWBPaper00006440
Curator_confirmedWBPerson2987
ReferenceWBPaper00005375
WBPaper00003645
WBPaper00022103
WBPaper00006440
WBPaper00002870
WBPaper00005116
WBPaper00003574
MethodAllele