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WormBase Tree Display for Variation: WBVar00145532

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Name Class

WBVar00145532NamePublic_namegk125
HGVSgCHROMOSOME_V:g.14017788_14018827del
Sequence_detailsSMapS_parentSequenceT08G5
Flanking_sequencescatatgagatcaaaagttagtaaatacttttttggatttttgaaaataaaatttgataga
Mapping_targetT08G5
Type_of_mutationInsertiona
Deletion
PCR_productGK125_external
GK125_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00035525
WBStrain00040877
LaboratoryVC
PersonWBPerson427
KO_consortium_allele
StatusLive
AffectsGeneWBGene00003474
WBGene00011622
TranscriptT08G5.10.1VEP_consequencetranscript_ablation
VEP_impactHIGH
Intron_number2/3
Exon_number1-4/4
T08G5.1.1VEP_consequence5_prime_UTR_variant
VEP_impactMODIFIER
cDNA_position?-62
Exon_number1/7
InteractorWBInteraction000524545
WBInteraction000524569
WBInteraction000524570
GeneticsMapping_dataIn_multi_point4500
DescriptionPhenotypeWBPhenotype:0000043Paper_evidenceWBPaper00024351
Curator_confirmedWBPerson2987
Remarkmtl-2(gk125) mutant animals showed a modest but significant increase in 'egg-to-egg' generation time compared to wild type animals (Table 1).Paper_evidenceWBPaper00024351
Curator_confirmedWBPerson2987
WBPhenotype:0000137Paper_evidenceWBPaper00034661
Curator_confirmedWBPerson2987
RemarkThe mtl-2(gk125) mutation resulted in reduced induction of mRNA expression of the genes M162.5 and sod-3 in response to silver nanoparticle (AgNP) exposure, compared to wild type animals and as determined by qRT-PCR (Figure 4 compared to Figure 3A).Paper_evidenceWBPaper00034661
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00005288Paper_evidenceWBPaper00034661
Curator_confirmedWBPerson2987
WBPhenotype:0000154Paper_evidenceWBPaper00028866
Curator_confirmedWBPerson2987
RemarkFigure 2Paper_evidenceWBPaper00028866
Curator_confirmedWBPerson2987
WBPhenotype:0000357Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
RemarkA high number and an earlier onset of unfertilized embryos (oocytes) were observed in mtl-2(gk125) mutants compared with wild type (data not shown).Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
WBPhenotype:0000519Paper_evidenceWBPaper00033444
Curator_confirmedWBPerson2021
RemarkMutant strains tested showed altered fitness compared to wild type when exposed to different bacterial environments such as E. coli, M. luteus, and Pseudomonas sp.and B. megateriumPaper_evidenceWBPaper00033444
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00033444
Curator_confirmedWBPerson2021
WBPhenotype:0000591Paper_evidenceWBPaper00034661
Curator_confirmedWBPerson2987
Remarkmtl-2(gk125) mutants exhibited a significant resistance to the toxic effects of silver nanoparticles on reproduction (Table 1)Paper_evidenceWBPaper00034661
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00005288Paper_evidenceWBPaper00034661
Curator_confirmedWBPerson2987
WBPhenotype:0001655Paper_evidenceWBPaper00024351
WBPaper00041036
Curator_confirmedWBPerson2987
Remarkmtl-2(gk125) mutant animals showed a modest decrease in life span in the presence of 30 μM cadmium compared to wild type animals (Figure 6)Paper_evidenceWBPaper00024351
Curator_confirmedWBPerson2987
mtl-2(gk125) mutants exhibited a significantly reduced growth rate in the presence of 100 μM cadmium, compared to wild type (Figure 5)Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003022Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
WBPhenotype:0002125Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
RemarkExposure to selected coated silver nanoparticles (Ag NPs), PVP8, PVP38, CIT7, GA5 and GA22 resulted in an enhanced level of growth inhibition compared to N2 animals exposed to the same nanoparticles and silver nitrate.Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005288Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005290Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005293Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005294Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005295Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00005296Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBMol:00003522Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson712
WBPhenotype:0002230Paper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
RemarkIn the presence of 25 μM cadmium, mtl-2(gk125) mutants exhibited increased accumulation of cadmium, compared to wild type controls (Figure 4A, Table S1), as determined by inductively coupled plasma optical emission spectrometry (ICP-OES)Paper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
In the presence of 340 μM zinc, mtl-2(gk125) mutants exhibited substantially increased accumulation of zinc, compared to wild type controls (Figure 4B, Table S1), as determined by inductively coupled plasma optical emission spectrometry (ICP-OES)Paper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003022Paper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
WBMol:00005064Paper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAnimals were exposed to 25 micromolar cadmiumPaper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
Animals were exposed to 340 micromolar zincPaper_evidenceWBPaper00036389
Curator_confirmedWBPerson2987
WBPhenotype:0002251Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
RemarkExposure to selected coated silver nanoparticles (Ag NPs), PVP8, PVP38, CIT7, GA5 and GA22 resulted in an enhanced level of growth inhibition compared to N2 animals exposed to the same nanoparticles and silver nitrate.Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
Affected_byMoleculeWBMol:00005288Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
WBMol:00005290Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
WBMol:00005293Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
WBMol:00005294Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
WBMol:00005295Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
WBMol:00005296Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
WBMol:00003522Paper_evidenceWBPaper00040519
Curator_confirmedWBPerson557
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00028866
Curator_confirmedWBPerson2987
RemarkFigure 4Paper_evidenceWBPaper00028866
Curator_confirmedWBPerson2987
WBPhenotype:0000050Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
RemarkSupplementary Table 2Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
WBPhenotype:0000673Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
RemarkSupplementary Table 2Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
Phenotype_assayTemperature20Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
WBPhenotype:0001175Paper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
Remarkdata not shownPaper_evidenceWBPaper00041036
Curator_confirmedWBPerson2987
WBPhenotype:0001379Paper_evidenceWBPaper00044243
Curator_confirmedWBPerson3701
Remarknot more sensitive than wildtype to toxicity from exposure to pore water from sediments downstream of mountaintop mining sites (Figure S2)Paper_evidenceWBPaper00044243
Curator_confirmedWBPerson3701
WBPhenotype:0001653Paper_evidenceWBPaper00028866
WBPaper00033210
Curator_confirmedWBPerson2987
RemarkFigure 2, Figure 4Paper_evidenceWBPaper00028866
Curator_confirmedWBPerson2987
mtl-2(gk125) mutants exhibited a normal decrease in cystathionine levels and increase in phytochelatin levels in response to cadmium exposure, compared to wild type controls (Figure 2, 4)Paper_evidenceWBPaper00033210
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003022Paper_evidenceWBPaper00033210
Curator_confirmedWBPerson2987
ReferenceWBPaper00040519
WBPaper00041036
WBPaper00034661
WBPaper00033444
WBPaper00024351
WBPaper00028866
WBPaper00033210
WBPaper00036389
WBPaper00044243
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele