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WormBase Tree Display for Variation: WBVar00145675

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Name Class

WBVar00145675EvidencePaper_evidenceWBPaper00035961
NamePublic_namegk268
HGVSgCHROMOSOME_III:g.675515_677052del
Sequence_detailsSMapS_parentSequenceW02B3
Flanking_sequencesttccattccccctcctttcccaagtcagtagcacggtcttaagcataaaaaataagttga
Mapping_targetW02B3
Type_of_mutationDeletion
PCR_productgk268_external
gk268_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00007481
WBStrain00030533
WBStrain00035834
WBStrain00055927
WBStrain00055928
WBStrain00055929
WBStrain00055933
LaboratoryVC
JN
PersonWBPerson427
KO_consortium_allele
StatusLive
AffectsGeneWBGene00001709
WBGene00196366
TranscriptW02B3.2.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
VEP_impactHIGH
Intron_number2-4/10
Exon_number1-4/11
W02B3.8VEP_consequencetranscript_ablation
VEP_impactHIGH
Exon_number1/1
Interactor (39)
IsolationMutagenTMP/UV
DescriptionPhenotype (16)
Phenotype_not_observedWBPhenotype:0000274Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Remark"Because we did not observe a significant number of unfertilized eggs or dead embryos in the grk-2 mutant strains, this suggests that the sperm capacity and development of embryos are normal in these strains."Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
WBPhenotype:0000357Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Remark"Because we did not observe a significant number of unfertilized eggs or dead embryos in the grk-2 mutant strains, this suggests that the sperm capacity and development of embryos are normal in these strains."Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
WBPhenotype:0000424Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Remark"To determine whether there were any changes in 5-HT synthesis, we measured tph-1 expression in N2 and grk-2 mutants by generating transgenic strains expressing GFP using the tph-1 promoter (tph-1::GFP). No differences in tph-1::GFP expression were observed in the N2 and grk-2 mutant strains as assessed by fluorescence (data not shown). We also evaluated TPH-1 using a mammalian Tph1 antibody and found that TPH-1 protein levels are similar in wild type and grk-2 mutants at several developmental stages (Fig. 4B)."Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
WBPhenotype:0000673Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Remark"Although there was no difference in the brood size between N2 and grk-2 mutants (Fig. 1D), a significant number of the laid eggs were late stage in the grk-2 loss-of-function strains (Fig. 1E)."Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00050796
Curator_confirmedWBPerson2987
ReferenceWBPaper00035961
WBPaper00050796
WBPaper00053144
WBPaper00064927
WBPaper00066032
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele