WormBase Tree Display for Variation: WBVar00145734

WBVar00145734 Evidence: Paper_evidence: WBPaper00032243
  Name: Public_name: gk327
    Other_name: CE20184:p.Ser107_Ter156delextTer?
      Y6B3B.10.1:c.318_467del
    HGVSg: CHROMOSOME_I:g.13726938_13727137del
  Sequence_details: SMap: S_parent: Sequence: Y6B3B
    Flanking_sequences: aacgccgattgaactgcatatatcacttgt tcgggtactttatgggcaaatcgggggtat
    Mapping_target: Y6B3B
    Type_of_mutation: Deletion
    PCR_product: gk327_external
      gk327_internal
    SeqStatus: Sequenced
    Deletion_verification: PCR with one primer internal to the deletion and one external confirms that no WT copy of the gene remains. Person_evidence: WBPerson154
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00036034
      WBStrain00040672
      WBStrain00040673
      WBStrain00040674
      WBStrain00040675
    Laboratory: VC
    Person: WBPerson427
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00006505
    Transcript: Y6B3B.10.1 (11)
    Interactor: WBInteraction000503803
      WBInteraction000535461
  Isolation: Mutagen: TMP/UV
  Description: Phenotype: WBPhenotype:0000436 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: The reduction in CED-4 mitochondrial colocalization and the accompanying increase in nuclear CED-4 signal upon irradiation was blocked entirely. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005175 PATO:0000460 Paper_evidence: WBPaper00032243
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were scored for CED-4 localization at 36 hours postradiation. Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
          Genotype: opIs219 Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
      WBPhenotype:0000717 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: 120-Gy-induced p53-mediated egl-1 (left) and ced-13 (right) up-regulation were not affected, as measured by reverse transcription polymerase chain reaction. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
      WBPhenotype:0001172 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: Age-related and radiation-induced germ cell apoptosis were nearly abolished compared to wild-type animals. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: L3-L4 larvae were assayed. Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
      WBPhenotype:0002541 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: Radiation-induced germ cell apoptosis was inhibited. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005784 PATO:0000460 Paper_evidence: WBPaper00032243
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: At least 20 animals were scored for germ cell apoptosis at 36 hours post-120 Gy and compared to WT-irradiated controls. Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000648 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: Mating efficiency was not significantly different from wild type. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: For each strain, 16 males were mated with 16 late L4 stage unc-51 hermaphrodites for 24 hours and then scored for cross progeny (non-Dpy progeny) and self progeny. Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
      WBPhenotype:0000673 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: Brood size was not significantly different from wild type. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
      WBPhenotype:0000812 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: The number of germ cell nuclei, 325.2 17.4 (n=10), was slightly less than wild type, 366.6 14.6 (n=10). Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005175 PATO:0000460 Paper_evidence: WBPaper00032243
                Curator_confirmed: WBPerson712
      WBPhenotype:0000885 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: The rate of germ cell corpse removal was unaffected. Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: L3-L4 larvae were assayed. Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
      WBPhenotype:0000965 Paper_evidence: WBPaper00032243
        Curator_confirmed: WBPerson712
        Remark: 0.1 0.4 (n=30) extra cells were counted in the anterior pharynx, similar to wild-type animals 0.05 0.22 (n=20). Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00032243
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: L3-L4 larvae were assayed. Paper_evidence: WBPaper00032243
              Curator_confirmed: WBPerson712
  Reference: WBPaper00032243
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele