WormBase Tree Display for Variation: WBVar00250120

WBVar00250120 Name: Public_name: tm1103
    Other_name: F55B12.1.1:c.375_481delinsATGTACGTT
      CE25006:p.Leu126CysfsTer27
    HGVSg: CHROMOSOME_V:g.13814221_13814711delinsATGTACGTT
  Sequence_details: SMap: S_parent: Sequence: F55B12
    Flanking_sequences: gaatgatccaagatttgcagcagctgcagc aagtttacgaattggaacgaagattcaaac
    Mapping_target: F55B12
    Source_location: 7 CHROMOSOME_V 13814220 13814712 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Insertion: ATGTACGTT
      Deletion
    PCR_product: tm1103_external
      tm1103_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00008317
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 1103
    NBP_allele
    Status: Live
  Affects: Gene: WBGene00000447
    Transcript: F55B12.1.1 (11)
  Isolation: Mutagen: TMP/UV
  Genetics: Map: V
  Description: Phenotype: WBPhenotype:0000572 Paper_evidence: WBPaper00050847
        Curator_confirmed: WBPerson38423
        Remark: "In ceh-24 mutant worms, expression from the ceh-24 promoter was increased. Processes did not run straight along the muscle but diverted from it or branched. Processes also ended prematurely and did not extend as far posteriorly as in the wild type (Figure 3B)" Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Variation_effect: Null: Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        EQ_annotations: Anatomy_term: WBbt:0005361 PATO:0000460 Paper_evidence: WBPaper00050847
                Curator_confirmed: WBPerson38423
            WBbt:0005359 PATO:0000460 Paper_evidence: WBPaper00050847
                Curator_confirmed: WBPerson38423
            WBbt:0005353 PATO:0000460 Paper_evidence: WBPaper00050847
                Curator_confirmed: WBPerson38423
        Phenotype_assay: Strain: WBStrain00008317 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Genotype: goeIs247[pceh-24::GCaMP6s::mKate2-unc-54-3'utr, unc-119(-)] Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
      WBPhenotype:0000795 Paper_evidence: WBPaper00050847
        Curator_confirmed: WBPerson38423
        Remark: reduced flipping behavior Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Variation_effect: Null: Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Phenotype_assay: Strain: WBStrain00008317 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
      WBPhenotype:0002367 Paper_evidence: WBPaper00050847
        Curator_confirmed: WBPerson38423
        Remark: "ceh-24 mutant worms could also eventually escape from the indentation, but needed four times longer for this (Figure 2E)" Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Variation_effect: Null: Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Phenotype_assay: Strain: WBStrain00008317 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
    Phenotype_not_observed: WBPhenotype:0000077 Paper_evidence: WBPaper00050847
        Curator_confirmed: WBPerson38423
        Remark: There is no contribution of flipping to the molting process. Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Variation_effect: Null: Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Phenotype_assay: Strain: WBStrain00008317 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
      WBPhenotype:0000315 Paper_evidence: WBPaper00050847
        Curator_confirmed: WBPerson38423
        Remark: "ceh-24(-) responded normally to mechanical stimulation (Figure 2 - figure supplement 2C)" Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Variation_effect: Null: Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Phenotype_assay: Strain: WBStrain00008317 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
      WBPhenotype:0001524 Paper_evidence: WBPaper00050847
        Curator_confirmed: WBPerson38423
        Remark: "locomotion quiescence during sleep appeared normal"; "Despite the absence of flipping, the time the animals took from resuming pumping until complete shedding of the old cuticle was not significantly altered in ceh-24 mutant animals and we could not detect any obvious defects in the new cuticle (Figure 2D)" Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Variation_effect: Null: Paper_evidence: WBPaper00050847
            Curator_confirmed: WBPerson38423
        Phenotype_assay: Strain: WBStrain00008317 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
          Control_strain: WBStrain00000001 Paper_evidence: WBPaper00050847
              Curator_confirmed: WBPerson38423
  Reference: WBPaper00050847
  Remark: 4519/4520-ATGTACGTT-5010/5011 (490 bp deletion + 8 bp insertion)
    This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
  Method: NBP_knockout_allele