Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00250731

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00250731NamePublic_nametm1766
Other_nameY4C6A.2d.1:c.505-222_783-59del
Y4C6A.2a.1:c.505-222_783-59del
HGVSgCHROMOSOME_IV:g.5441508_5442315del
Sequence_detailsSMapS_parentSequenceY4C6A
Flanking_sequencesgaaattagcaaaaagcgaaacataacattcgtaccccaaatttcaaagagaaatcatgaa
Mapping_targetY4C6A
Source_location7CHROMOSOME_IV54415075442316Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm1766_external
tm1766_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq1766
NBP_allele
StatusLive
AffectsGeneWBGene00048429
WBGene00021152
WBGene00165129
TranscriptY4C6A.14
Y4C6A.21
Y4C6A.2a.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScY4C6A.2a.1:c.505-222_783-59del
Intron_number3-5/19
Exon_number4-5/20
Y4C6A.2d.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScY4C6A.2d.1:c.505-222_783-59del
Intron_number3-5/19
Exon_number4-5/20
IsolationMutagenTMP/UV
GeneticsMapIV
DescriptionPhenotypeWBPhenotype:0000016Paper_evidenceWBPaper00060924
Curator_confirmedWBPerson557
Phenotype_assayTreatmentOne day old adult worms were placed on Aldicarb (0.1 mM) plates.Paper_evidenceWBPaper00060924
Curator_confirmedWBPerson557
Temperature20Paper_evidenceWBPaper00060924
Curator_confirmedWBPerson557
WBPhenotype:0000420Paper_evidenceWBPaper00060924
Curator_confirmedWBPerson557
Phenotype_assayTreatmentLevamisole assays were carried out on NGM plates with 200 M Levamisole.Paper_evidenceWBPaper00060924
Curator_confirmedWBPerson557
Temperature20Paper_evidenceWBPaper00060924
Curator_confirmedWBPerson557
Phenotype_not_observedWBPhenotype:0000006Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkAll these animals retained a similar number of eggs when compared to wild-type animals (~10 eggs).Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentYoung well-fed animals were scoredPaper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature25Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0000019Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkAnimals did not exhibit reduced pharyngeal pumping when compared with daf-7(e1375).Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentPumping rates were assayed for well-fed , pheromone-untreated; food-deprived, pheromone-untreated; and well-fed, pheromone-treated animals. Pheromone treatment consisted of transfer of animals grown under well-fed conditions to plates containing 30 ul/ml of a dauer-pheromone prep (Golden and Riddle, 1982) and 30 ml of OP50 E. coli food.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature20Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0000030Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComment to the National Bioresource Project of Japan from Dr. Y. Jin: grows well.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceCZ
WBPhenotype:0000062Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkClassified as homozygous viable by the National Bioresource of Japan.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
WBPhenotype:0000478Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComment from Dr. I. Mori to the National Bioresource Project of Japan: normal thermotaxis.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
WBPhenotype:0001182Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComment to the National Bioresource Project of Japan from Dr. K. Ashrafi: normal Sudan Black B staining.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceKQ
WBPhenotype:0001183Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
RemarkFat levels were reduced when compared to daf-7(e1375) as determined by Sudan Black assay (described in Kimura et al., 1997).Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Phenotype_assayTreatmentTo minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype.Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
Temperature22Paper_evidenceWBPaper00032082
Curator_confirmedWBPerson712
WBPhenotype:0001206Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComment to the National Bioresource Project of Japan from Dr. Y. Jin: no movement phenotype.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceCZ
Disease_infoModels_diseaseDOID:10652
Models_disease_in_annotationWBDOannot00001430
ReferenceWBPaper00032082
WBPaper00060924
Remark13745/13746-14553/14554 (808 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele