WormBase Tree Display for Variation: WBVar00251143

WBVar00251143 Name: Public_name: tm2217
    Other_name: C08F8.8.1:c.367-230_807-48del
    HGVSg: CHROMOSOME_IV:g.11172919_11174002del
  Sequence_details: SMap: S_parent: Sequence: C08F8
    Flanking_sequences: tattctggggcagatgttttaatgtttcta gtaccatctttccttctgaaaacttcacag
    Mapping_target: C08F8
    Source_location: 7 CHROMOSOME_IV 11172918 11174003 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Deletion
    PCR_product: tm2217_external
      tm2217_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00027634
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 2217
    NBP_allele
    Status: Live
  Affects: Gene: WBGene00003657
    Transcript: C08F8.8.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: C08F8.8.1:c.367-230_807-48del
        Intron_number: 5-7/9
        Exon_number: 6-7/10
  Isolation: Mutagen: TMP/UV
  Genetics: Map: IV
  Description: Phenotype: WBPhenotype:0000006 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: All tm2217 homozygous escaper adults had egg-laying defective (Egl) phenotypes. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000062 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson48
        Remark: Classified as lethal OR sterile by the National Bioresource Project of Japan. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson48
            Laboratory_evidence: FX
      WBPhenotype:0000073 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Arrested L1 larvae displayed variable defects in tail morphology, including kinks, bulges, and forks. Tail morphology defects were not observed in later stage escapers. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000081 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Homozygous mutants usually arrested development as embryos or L1 larvae. Less than 10% of tm2217 homozygous animals developed to adulthood. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
        EQ_annotations: Life_stage: WBls:0000024 PATO:0000460 Paper_evidence: WBPaper00039839
                Curator_confirmed: WBPerson712
      WBPhenotype:0000154 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: All tm2217 homozygous escaper adults produced a small number (1-5) of progeny that invariably arrested at or before the L1 stage. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000643 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Arrested L1 appeared slightly uncoordinated in comparison to heterozygous sisters. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000688 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson48
        Remark: Classified as lethal OR sterile by the National Bioresource Project of Japan. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson48
            Laboratory_evidence: FX
      WBPhenotype:0000697 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: All tm2217 homozygous escaper adults had protruding vulva (Pvl). Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000708 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Arrested L1 larvae had grossly normal intestines. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000709 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Arrested L1 larvae had grossly normal pharynxes. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000778 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Over 90% of arrested larvae did not have any bacteria in their gut, suggesting that most nhr-67 homozygous mutantsare unable to eat. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0000867 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Homozygous mutants usually arrested development as embryos or L1 larvae. Less than 10% of tm2217 homozygous animals developed to adulthood. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
      WBPhenotype:0001412 Paper_evidence: WBPaper00039839
        Curator_confirmed: WBPerson712
        Remark: Arrested L1 larvae had grossly normal cuticular alae. Paper_evidence: WBPaper00039839
            Curator_confirmed: WBPerson712
  Reference: WBPaper00039839
  Remark: 24339/24340-25423/25424 (1081 bp deletion)
    This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
  Method: NBP_knockout_allele