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WormBase Tree Display for Variation: WBVar00252888

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Name Class

WBVar00252888NamePublic_nametm4391
Other_nameC34G6.5.1:c.375_589del
CE26909:p.Tyr126ArgfsTer10
HGVSgCHROMOSOME_I:g.5872746_5873063del
Sequence_detailsSMapS_parentSequenceC34G6
Flanking_sequencesttcaaaacgagatctttcatttgctctctcaaggtacatcgtcaccgtgtatgttttgta
Mapping_targetC34G6
Source_location7CHROMOSOME_I58727455873064Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm4391_external
tm4391_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00047819
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq4391
NBP_allele
StatusLive
AffectsGeneWBGene00016421
TranscriptC34G6.5.1 (11)
IsolationMutagenTMP/UV
GeneticsMapI
DescriptionPhenotypeWBPhenotype:0000031Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkClassified as Gro by the National Bioresource Project of JapanPerson_evidenceWBPerson7743
Curator_confirmedWBPerson712
WBPhenotype:0000688Paper_evidenceWBPaper00055989
Curator_confirmedWBPerson712
Remarkcdc-7(tm4391) worms are temperature sensitive sterile, with higher temperatures being progressively worse for fecundity. At 25 degC, 90% of individual worms tested were sterile (Total n= 48. Number of plates with progeny = 3, number without progeny = 45). There was an intermediate effect at 20 degC with 56% sterility (Total n= 49. Number of plates with progeny = 21, number without progeny = 28). cdc-7(tm4391) was most fertile when kept at 16 degC, with only 30% sterility (Total n= 49. Number of plates with progeny = 35, number without progeny = 14). N2 controls had no observed sterility at any temperatures (for 25 degC, 20 degC and 16 degC respectively, total n= 50, 50, 50. Number of plates with progeny = 50, 50, 50).Paper_evidenceWBPaper00055989
Curator_confirmedWBPerson712
ImageWBPicture0000014384Paper_evidenceWBPaper00055989
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive20, 25Paper_evidenceWBPaper00055989
Curator_confirmedWBPerson712
Phenotype_assayTemperature16, 22, 25Paper_evidenceWBPaper00055989
Curator_confirmedWBPerson712
GenotypeCK609 CK609 cdc-7(tm4391) I - outcrossed 4x to N2Paper_evidenceWBPaper00055989
Curator_confirmedWBPerson712
WBPhenotype:0002327Paper_evidenceWBPaper00046077
Curator_confirmedWBPerson3637
Remarksuppressors of TDP-43 transgene induced uncoordinationPaper_evidenceWBPaper00046077
Curator_confirmedWBPerson3637
Disease_infoModifies_diseaseDOID:332
DOID:9255
DOID:0060201
Modifies_disease_in_annotationWBDOannot00000773
WBDOannot00000776
WBDOannot00000785
ReferenceWBPaper00046077
WBPaper00055989
Remark2726/2727-3044/3045 (318 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele