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WormBase Tree Display for Variation: WBVar00275123

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Name Class

WBVar00275123EvidencePaper_evidenceWBPaper00005233
NamePublic_namewa17
Sequence_detailsMapping_targetW02A2
Type_of_mutationSubstitution
SeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00004011
LaboratoryBX
StatusLive
AffectsGeneWBGene00001394
InteractorWBInteraction000504723
GeneticsMapping_dataIn_multi_point4298
DescriptionPhenotypeWBPhenotype:0000031Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
RemarkThe loss of normal polyunsaturated fatty acids (PUFAs) in fat-2 homozygotes causes many defects in this strain including slow growth, abnormal body shape, sluggish movement, cuticle defects, and reduced brood size. After 3 days of growth, 2-fold embryos produced by fat-2 homozygotes developed to the L2 larval stage, whereas similar staged WT embryos reached adulthood and already were laying eggs (Fig. 3A and C). We found that 7 days at 20 degrees Celsius were required before the 2-fold fat-2 embryos reached adulthood.Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
WBPhenotype:0000072Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
RemarkThe loss of normal polyunsaturated fatty acids (PUFAs) in fat-2 homozygotes causes many defects in this strain including slow growth, abnormal body shape, sluggish movement, cuticle defects, and reduced brood size. After 3 days of growth, 2-fold embryos produced by fat-2 homozygotes developed to the L2 larval stage, whereas similar staged WT embryos reached adulthood and already were laying eggs (Fig. 3A and C). We found that 7 days at 20 degrees Celsius were required before the 2-fold fat-2 embryos reached adulthood.Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
WBPhenotype:0000138Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
RemarkThe fatty acid composition of fat-2 mutants differs remarkably from that of WT. These mutants were detected in F1 segregating populations because of a noticeable increase in the amount of 18:1n9 fatty acid, which increases from 2.6 to 7%, whereas the amounts of 18:2n6 and C20 polyunsaturated fatty acids (PUFAs) are reduced slightly. Analysis of segregating populations revealed that the homozygous strain was incapable of producing the normal levels of C18 or C20 PUFAs (Fig. 3C, Table 1). It is likely that thewa17 strain is capable of carrying out a small amount of delta-12 desaturase activity (~5% of WT activity), because authors detected a small amount of 20:5n3 (1.9%) in these worms compared with 39.6% of delta-12 desaturated PUFAs in WT (Table 1). In fat-2 homozygous worms, 18:1n9 accumulates nearly 10-fold above that found in WT to 24.3%. The animals are not completely devoid of PUFAs, however. In the absence of the delta-12 desaturase, small peaks of unusual 18:2 and 20:2 were identified. Analysis of 4,4-dimethyloxazoline derivatives of these fatty acids by GC mass spectroscopy indicated the presence of 18:2n9 (18:2-delta-6,9), 18:2n7(delta-8,11), and 20:2n9(delta-8,11) (Fig. 3C).Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
WBPhenotype:0000154Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
RemarkThe loss of normal polyunsaturated fatty acids (PUFAs) in fat-2 homozygotes causes many defects in this strain including slow growth, abnormal body shape, sluggish movement, cuticle defects, and reduced brood size. After 3 days of growth, 2-fold embryos produced by fat-2 homozygotes developed to the L2 larval stage, whereas similar staged WT embryos reached adulthood and already were laying eggs (Fig. 3A and C). We found that 7 days at 20 degrees Celsius were required before the 2-fold fat-2 embryos reached adulthood.Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
WBPhenotype:0000201Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
RemarkThe loss of normal polyunsaturated fatty acids (PUFAs) in fat-2 homozygotes causes many defects in this strain including slow growth, abnormal body shape, sluggish movement, cuticle defects, and reduced brood size. After 3 days of growth, 2-fold embryos produced by fat-2 homozygotes developed to the L2 larval stage, whereas similar staged WT embryos reached adulthood and already were laying eggs (Fig. 3A and C). We found that 7 days at 20 degrees Celsius were required before the 2-fold fat-2 embryos reached adulthood.Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
WBPhenotype:0000646Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
RemarkThe loss of normal polyunsaturated fatty acids (PUFAs) in fat-2 homozygotes causes many defects in this strain including slow growth, abnormal body shape, sluggish movement, cuticle defects, and reduced brood size. After 3 days of growth, 2-fold embryos produced by fat-2 homozygotes developed to the L2 larval stage, whereas similar staged WT embryos reached adulthood and already were laying eggs (Fig. 3A and C). We found that 7 days at 20 degrees Celsius were required before the 2-fold fat-2 embryos reached adulthood.Paper_evidenceWBPaper00005233
Curator_confirmedWBPerson2987
WBPhenotype:0001720Paper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
RemarkIn mutant hermaphrodites, sperm are often found throughout the uterus and near the vulva unlike wild-type. Many of these sperm are likely ejected into the external environment during egg laying, causing a reduction in fertilized egg productionPaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentWild-type males labelled with MitoTracker were mated to non-labelled mutant hermaphrodites or were subjected to DAPI stainingPaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
WBPhenotype:0001823Paper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
RemarkMale sperm failed to accumulate in the spermathecaPaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentWild-type males labelled with MitoTracker were mated to non-labelled mutant hermaphroditesPaper_evidenceWBPaper00028527
Curator_confirmedWBPerson2021
ReferenceWBPaper00005233
WBPaper00028527
MethodSubstitution_allele