WormBase Tree Display for Variation: WBVar00275146

WBVar00275146 Evidence: Paper_evidence: WBPaper00044867
  Name: Public_name: wk7
    Other_name: C32D5.2.1:c.216T>A
      CE01842:p.Tyr72Ter
    HGVSg: CHROMOSOME_II:g.6324751T>A
  Sequence_details: SMap: S_parent: Sequence: C32D5
    Flanking_sequences: tattttgaaaaatcgatgttattgcaatta gatcagtcgatatgtggaaataatatgaca
    Mapping_target: C32D5
    Type_of_mutation: Substitution: t r Paper_evidence: WBPaper00044867
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00026327
    Laboratory: LT
    Status: Live
  Affects: Gene: WBGene00004860
    Transcript: C32D5.2.1 VEP_consequence: stop_gained
        VEP_impact: HIGH
        HGVSc: C32D5.2.1:c.216T>A
        HGVSp: CE01842:p.Tyr72Ter
        cDNA_position: 221
        CDS_position: 216
        Protein_position: 72
        Exon_number: 3/14
        Codon_change: taT/taA
        Amino_acid_change: Y/*
    Interactor: WBInteraction000500850
      WBInteraction000500860
      WBInteraction000502075
      WBInteraction000504396
      WBInteraction000544073
      WBInteraction000555971
  Genetics: Interpolated_map_position: II -0.382225
  Description: Phenotype: WBPhenotype:0000070 Paper_evidence: WBPaper00005858
        Curator_confirmed: WBPerson712
        Remark: Tale has fusions of rays 4-5,6-7,8-9. Paper_evidence: WBPaper00005858
            Curator_confirmed: WBPerson712
      WBPhenotype:0000136 Paper_evidence: WBPaper00025090
        Curator_confirmed: WBPerson2021
        Remark: Mutants show an up-regulation of the lon-1 transcript Paper_evidence: WBPaper00025090
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000154 Paper_evidence: WBPaper00025090
        Curator_confirmed: WBPerson2021
        Remark: Mutants show a reduction in brood size Paper_evidence: WBPaper00025090
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000229 Paper_evidence: WBPaper00029002
          WBPaper00036735
          WBPaper00005858
        Curator_confirmed: WBPerson557
          WBPerson712
      WBPhenotype:0000297 Paper_evidence: WBPaper00036735
        Curator_confirmed: WBPerson712
        Remark: Although structural processes were fused, neuronal processes were well separated. Paper_evidence: WBPaper00036735
            Curator_confirmed: WBPerson712
      WBPhenotype:0000420 Paper_evidence: WBPaper00034730
        Curator_confirmed: WBPerson2021
        Remark: sma-6(wk7) animals were hypersensitive to levamisole Paper_evidence: WBPaper00034730
            Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00004019 Paper_evidence: WBPaper00034730
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000901 Paper_evidence: WBPaper00045065
        Curator_confirmed: WBPerson460
      WBPhenotype:0001183 Paper_evidence: WBPaper00053184
        Curator_confirmed: WBPerson12433
      WBPhenotype:0001202 Paper_evidence: WBPaper00034730
        Curator_confirmed: WBPerson2021
        Remark: sma-6(wk7) animals were hypersensitive to nicotine Paper_evidence: WBPaper00034730
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001212 Paper_evidence: WBPaper00034730
        Curator_confirmed: WBPerson2021
        Remark: sma-6(wk7) mutants were sick and paralyzed immediately in drug assays, likely indicating a cuticle defect Paper_evidence: WBPaper00034730
            Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000006 Paper_evidence: WBPaper00032082
        Curator_confirmed: WBPerson712
        Remark: All these animals retained a similar number of eggs when compared to wild-type animals (~10 eggs). Paper_evidence: WBPaper00032082
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Young well-fed animals were scored Paper_evidence: WBPaper00032082
              Curator_confirmed: WBPerson712
          Temperature: 25 Paper_evidence: WBPaper00032082
              Curator_confirmed: WBPerson712
      WBPhenotype:0000019 Paper_evidence: WBPaper00032082
        Curator_confirmed: WBPerson712
        Remark: Animals did not exhibit reduced pharyngeal pumping when compared with daf-7(e1375). Paper_evidence: WBPaper00032082
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Pumping rates were assayed for well-fed , pheromone-untreated; food-deprived, pheromone-untreated; and well-fed, pheromone-treated animals. Pheromone treatment consisted of transfer of animals grown under well-fed conditions to plates containing 30 ul/ml of a dauer-pheromone prep (Golden and Riddle, 1982) and 30 ml of OP50 E. coli food. Paper_evidence: WBPaper00032082
              Curator_confirmed: WBPerson712
          Temperature: 20 Paper_evidence: WBPaper00032082
              Curator_confirmed: WBPerson712
      WBPhenotype:0001182 Paper_evidence: WBPaper00032082
        Curator_confirmed: WBPerson712
        Remark: Fat levels were similar to that seen in wild-type but are reduced when compared to daf-7(e1375) as determined by Sudan Black assay (described in Kimura et al., 1997). Paper_evidence: WBPaper00032082
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: To minimize staining variability, which allowed for quantitative comparisons between various genotypes: animals from one genotype were labeled with fluorescein isothiocyanate (FITC) and then fixed and stained in the same tube as unlabeled animals from another genotype. Paper_evidence: WBPaper00032082
              Curator_confirmed: WBPerson712
          Temperature: 22 Paper_evidence: WBPaper00032082
              Curator_confirmed: WBPerson712
      WBPhenotype:0001184 Paper_evidence: WBPaper00032966
        Curator_confirmed: WBPerson2021
        Remark: Abnormally sized mutants did not display enhanced fat accumulation Paper_evidence: WBPaper00032966
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Nile Red staining Paper_evidence: WBPaper00032966
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001652 Paper_evidence: WBPaper00032446
        Curator_confirmed: WBPerson2021
  Reference (13)
  Method: Substitution_allele