WormBase Tree Display for Variation: WBVar00275305
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WBVar00275305 | Name | Public_name | y56 | ||||
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Sequence_details | SeqStatus | Pending_curation | |||||
Variation_type | Allele | ||||||
Origin | Species | Caenorhabditis elegans | |||||
Strain | WBStrain00006374 | ||||||
Laboratory | TY | ||||||
Status | Live | ||||||
Affects | Gene | WBGene00001086 | |||||
Description | Phenotype | WBPhenotype:0000066 | Paper_evidence | WBPaper00032450 | |||
Curator_confirmed | WBPerson712 | ||||||
Remark | Dosage compensation mutations cause XX specific maternal effect lethality. | Paper_evidence | WBPaper00032450 | ||||
Curator_confirmed | WBPerson712 | ||||||
Maternal | |||||||
WBPhenotype:0000718 | Paper_evidence | WBPaper00032450 | |||||
WBPaper00048953 | |||||||
Curator_confirmed | WBPerson712 | ||||||
WBPerson2987 | |||||||
Remark | Dosage compensation mutations cause XX specific maternal effect lethality. | Paper_evidence | WBPaper00032450 | ||||
Curator_confirmed | WBPerson712 | ||||||
"To test if the DCC represses X-chromosome expression shortly after localizing to the X, we collected dpy-27 null mutant embryos using a strain with genetically balanced (y56) allele (Materials and Methods). In early embryos, dpy-27 null mutation caused significant derepression of newly transcribed zygotic genes on the X (Fig 2E), suggesting that dpy-27 represses X chromosomes in early embryos." | Paper_evidence | WBPaper00048953 | |||||
Curator_confirmed | WBPerson2987 | ||||||
"To specifically study DCC mediated X repression, we analyzed gene expression changes in hermaphrodites mutant for dpy-27 or upon dpy-27 RNAi knockdown. As mutants and RNAi treated worms showed more variability in staging, we mainly used 'mixed stage embryos' isolated by bleaching gravid adults. Mixed stage embryos contained 100-300 cells (S4A Fig). We used dpy-27 RNAi in mixed embryos and L3, because of the difficulty collecting dpy-27(y56) null mutant. Western blot analyses showed ~70% and ~40% knockdown of DPY-27 in embryos and L3s, respectively (S4B Fig). Mutation (Fig 3D) or depletion (Fig 3E) of dpy-27 caused significant X chromosome derepression in early and mixed-stage embryos, L1 and L3 worms." | Paper_evidence | WBPaper00048953 | |||||
Curator_confirmed | WBPerson2987 | ||||||
Maternal | |||||||
WBPhenotype:0001403 | Paper_evidence | WBPaper00032450 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | CAPG-1 localizes to X chromosomes in wild-type hermphrodites, but not in animals carrying mutated subunits of condensin IDC. | Paper_evidence | WBPaper00032450 | ||||
Curator_confirmed | WBPerson712 | ||||||
Phenotype_not_observed | WBPhenotype:0000773 | Paper_evidence | WBPaper00032450 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Hermaphrodite and male progeny of mutant mothers showed chromosome segregation defects in many tissues, whereas dpy-27 mutants did not (scored for gut nuclei; Figure S6B), confirming that condensin I promotes mitotic chromosome segregation. | Paper_evidence | WBPaper00032450 | ||||
Curator_confirmed | WBPerson712 | ||||||
Reference | WBPaper00032450 | ||||||
WBPaper00048953 | |||||||
Method | Allele |