Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00275305

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00275305NamePublic_namey56
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00006374
LaboratoryTY
StatusLive
AffectsGeneWBGene00001086
DescriptionPhenotypeWBPhenotype:0000066Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
RemarkDosage compensation mutations cause XX specific maternal effect lethality.Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
Maternal
WBPhenotype:0000718Paper_evidenceWBPaper00032450
WBPaper00048953
Curator_confirmedWBPerson712
WBPerson2987
RemarkDosage compensation mutations cause XX specific maternal effect lethality.Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
"To test if the DCC represses X-chromosome expression shortly after localizing to the X, we collected dpy-27 null mutant embryos using a strain with genetically balanced (y56) allele (Materials and Methods). In early embryos, dpy-27 null mutation caused significant derepression of newly transcribed zygotic genes on the X (Fig 2E), suggesting that dpy-27 represses X chromosomes in early embryos."Paper_evidenceWBPaper00048953
Curator_confirmedWBPerson2987
"To specifically study DCC mediated X repression, we analyzed gene expression changes in hermaphrodites mutant for dpy-27 or upon dpy-27 RNAi knockdown. As mutants and RNAi treated worms showed more variability in staging, we mainly used 'mixed stage embryos' isolated by bleaching gravid adults. Mixed stage embryos contained 100-300 cells (S4A Fig). We used dpy-27 RNAi in mixed embryos and L3, because of the difficulty collecting dpy-27(y56) null mutant. Western blot analyses showed ~70% and ~40% knockdown of DPY-27 in embryos and L3s, respectively (S4B Fig). Mutation (Fig 3D) or depletion (Fig 3E) of dpy-27 caused significant X chromosome derepression in early and mixed-stage embryos, L1 and L3 worms."Paper_evidenceWBPaper00048953
Curator_confirmedWBPerson2987
Maternal
WBPhenotype:0001403Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
RemarkCAPG-1 localizes to X chromosomes in wild-type hermphrodites, but not in animals carrying mutated subunits of condensin IDC.Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000773Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
RemarkHermaphrodite and male progeny of mutant mothers showed chromosome segregation defects in many tissues, whereas dpy-27 mutants did not (scored for gut nuclei; Figure S6B), confirming that condensin I promotes mitotic chromosome segregation.Paper_evidenceWBPaper00032450
Curator_confirmedWBPerson712
ReferenceWBPaper00032450
WBPaper00048953
MethodAllele