Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar02146626

expand all nodes | collapse all nodes | view schema

Name Class

WBVar02146626EvidencePaper_evidenceWBPaper00049977
NamePublic_namemg570
Other_nameT19E7.2a.1:c.4_9delinsTAATGA
CE27591:p.Gly2_Gly3delinsTer
HGVSgCHROMOSOME_IV:g.5660376_5660381delinsTCATTA
Sequence_detailsSMapS_parentSequenceT19E7
Flanking_sequencescaggatctatcataatccagtcagtcgatgtcatcacgccgtcagcgaagtacgtcggcg
Mapping_targetT19E7
Type_of_mutationInsertionTAATGA
DeletionGGCGGT
SeqStatusSequenced
Variation_typeEngineered_allele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00007966
LaboratoryGR
Production_methodCRISPR_Cas9
StatusLive
AffectsGeneWBGene00004804
TranscriptT19E7.2a.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScT19E7.2a.1:c.4_9delinsTAATGA
HGVSpCE27591:p.Gly2_Gly3delinsTer
cDNA_position4-10
CDS_position4-10
Protein_position2-4
Exon_number1/9
Codon_changeGGCGGTTca/TAATGATca
Amino_acid_changeGGS/**S
DescriptionPhenotypeWBPhenotype:0000038Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
RemarkAge-associated vulval integrity defectPaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
WBPhenotype:0000039Paper_evidenceWBPaper00061439
Curator_confirmedWBPerson49407
WBPhenotype:0000135Paper_evidenceWBPaper00053771
Curator_confirmedWBPerson557
RemarkThere is an increase in gst-4mRNA levels in skn-1(mg570) mutants.Paper_evidenceWBPaper00053771
Curator_confirmedWBPerson557
WBPhenotype:0001013Paper_evidenceWBPaper00061439
Curator_confirmedWBPerson49407
WBPhenotype:0001171Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
Remarkreduced lifespanPaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
WBPhenotype:0001236Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Remarkincreased expression of mgIs77[rpl-28::ub(G76V)::GFP]; Failure to completely degrade ub(G76V)::GFPPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Phenotype_assayControl_strainWBStrain00007977Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
GenotypemgIs77 [rpl-28::ub(G76V)::GFP]Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
WBPhenotype:0001278Paper_evidenceWBPaper00049977
WBPaper00056554
WBPaper00057217
Curator_confirmedWBPerson5649
Remarkexpression of mgIs72[rpt-3::gfp] transgene reduced; Figure 2cPaper_evidenceWBPaper00049977
Curator_confirmedWBPerson5649
expression of mgIs72[rpt-3::gfp] transgene reduced. rpt-3::gfp expression is not induced in body wall of unc-54(mg519); skn-1(mg570) double mutantsPaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
expression of mgIs72[rpt-3::gfp] transgene reduced. rpt-3::gfp expression is not induced in body wall of unc-54(e1301); skn-1(mg570) double mutantsPaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
expression of mgIs72[rpt-3::gfp] transgene is not induced by amyloid beta (1-42) peptidePaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
induction of dvIs19[gst-4::gfp] by bortezomib reduced (Figure S6A)Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
reduced expression of mgIs72[rpt-3::gfp]; Basal expression of rpt-3::gfp reduced under standard culture conditionsPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
RecessivePaper_evidenceWBPaper00049977
Curator_confirmedWBPerson5649
Affected_byMoleculeWBMol:00003099Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Phenotype_assayControl_strainWBStrain00043199Paper_evidenceWBPaper00049977
Curator_confirmedWBPerson5649
WBStrain00043205Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
WBStrain00005102Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
WBStrain00007961Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Genotypepbs-5(mg502); mgIs72[rpt-3::gfp]Paper_evidenceWBPaper00049977
Curator_confirmedWBPerson5649
mgIs72[rpt-3::gfp]; unc-54(mg519)Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
mgIs72[rpt-3::gfp]; unc-54(e1301)Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
mgIs72 [rpt-3::gfp] II; dvIs2 [Punc-54::human Beta-peptide 1-42 minigene+ pRF4]Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
dvIs19 [gst-4::gfp]Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
mgIs72 [rpt-3::gfp]Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
WBPhenotype:0001405Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
RemarkAccumulation of Amyloid beta peptide increased. Increased accumulation of GFP::Abeta in day 2 adults compared to wild typePaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
Phenotype_assayControl_strainWBStrain00005104Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
GenotypedvIs37 [myo-3p::GFP::A-Beta (3-42) + rol-6(su1006)]Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
WBPhenotype:0001918Paper_evidenceWBPaper00049977
WBPaper00057217
Curator_confirmedWBPerson5649
Remarkincreased sensitivity to bortezomib; Figure 2h, bortezomib concentration that causes developmental arrest is ~100x lower than wild typePaper_evidenceWBPaper00049977
Curator_confirmedWBPerson5649
Increased sensitivity to bortezomib; Complete developmental arrest/larval lethality on plates containing 0.4 μg/ml bortezomibPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Increased sensitivity to bortezomib; >90% of adult animals die within 4 days on plates containing 0.04 μg/ml bortezomibPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Affected_byMoleculeWBMol:00003099Paper_evidenceWBPaper00049977
WBPaper00057217
Curator_confirmedWBPerson5649
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00049977
WBPaper00057217
Curator_confirmedWBPerson5649
Genotypewild typePaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
WBPhenotype:0001937Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
RemarkHuman amyloid beta (1-42) peptide toxicity enanced. Increased proportion of dvIs2 transgenic adults paralyzedPaper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
Phenotype_assayControl_strainWBStrain00005094Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
GenotypedvIs2 [Punc-54::human Beta-peptide 1-42 minigene+ pRF4]Paper_evidenceWBPaper00056554
Curator_confirmedWBPerson5649
Phenotype_not_observedWBPhenotype:0001278Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Remarkinduction of gst-4::gfp by juglone is not lost in mg570 mutantsPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
induction of gst-4::gfp by tkt-1(RNAi) is not lost in mg570 mutantsPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
induction of gst-4::gfp by wdr-23(RNAi) is not lost in mg570 mutantsPaper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Affected_byMoleculeWBMol:00004938Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Phenotype_assayControl_strainWBStrain00005102Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
GenotypedvIs19 [gst-4::gfp]Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
dvIs19 [gst-4::gfp]; tkt-1(RNAi)Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
dvIs19 [gst-4::gfp]; wdr-23(RNAi)Paper_evidenceWBPaper00057217
Curator_confirmedWBPerson5649
Disease_infoModifies_diseaseDOID:10652
Modifies_disease_in_annotationWBDOannot00000729
WBDOannot00000731
ReferenceWBPaper00049977
WBPaper00056554
WBPaper00057217
WBPaper00053771
WBPaper00061439
RemarkThis crispr allele actually introduces a double stop mutation G(2) to Ochre Stop and G(3) to Opal StopPerson_evidenceWBPerson5649
Manually curated Gene associations preserved as a text remark so that VEP is the canonical predictor of consequence: WBGene00004804 Ochre_UAA G(2) to StopPerson_evidenceWBPerson5649
MethodEngineered_allele