WormBase Tree Display for Variation: WBVar02149986
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WBVar02149986 | Evidence | Paper_evidence | WBPaper00057192 | ||||||
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Name | Public_name | cmp145 | |||||||
Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Laboratory | USC | ||||||||
Corresponding_transgene | WBTransgene00026135 | ||||||||
Status | Live | ||||||||
Description | Phenotype | WBPhenotype:0000306 | Paper_evidence | WBPaper00057192 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | "PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. " "the in vivo dynamics of PGL-1 foci are drastically altered when tagged with mKate2 or mTagBFP2 and appear to be more solid or aggregate-like than when tagged with GFP." "PGL-1::GFP condensates in embryos are observed to dissolve at temperature shifts to 34C for 1 minute (Putnam et al., 2019). In adult germlines, evidence of P granule de-mixing can be seen by 3 hours at 34C ,where PGL-1::GFP foci are faint and fluorescent signal is heavily cytoplasmic (D, top row). However, this same temperature shift results in large bright, abnormal foci and low cytoplasmic signal in both PGL-1::mKate2 and PGL-1::mTagBFP2 (D, middle row, bottom row). The majority of these foci appear detached from the nuclear periphery and large, round, abnormal aggregates are also observed in the syncytial gonad rachis (not shown)." | Paper_evidence | WBPaper00057192 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
"PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. " "the in vivo dynamics of PGL-1 foci are drastically altered when tagged with mKate2 or mTagBFP2 and appear to be more solid or aggregate-like than when tagged with GFP." " PGL1::GFP foci in the pachytene are disrupted 5 hours post-microinjection of -amanitin, a potent transcriptional inhibitor (E, top row). PGL-1::mKate2and PGL-1::mTagBFP2 both fail to dissolve, and instead form bright puncta that are abnormal compared to control foci (E, middle row, bottom row)." | Paper_evidence | WBPaper00057192 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Image | WBPicture0000014916 | Paper_evidence | WBPaper00057192 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004324 | Paper_evidence | WBPaper00057192 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005175 | PATO:0000460 | Paper_evidence | WBPaper00057192 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | 34 degC | Paper_evidence | WBPaper00057192 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Control_strain | WBStrain00042744 | Paper_evidence | WBPaper00057192 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Genotype | pgl-1(cmp145[pgl-1::mKate2::Lox2272::3xMyc]) IV | Paper_evidence | WBPaper00057192 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00057192 | ||||||||
Remark | Created in the nameserver by Karen Yook | ||||||||
WBPaper00057192 pgl-1 [pgl-1::mKate2::Lox2272::3xMyc]) IV | |||||||||
Method | Allele |