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WormBase Tree Display for Variation: WBVar02149986

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Name Class

WBVar02149986EvidencePaper_evidenceWBPaper00057192
NamePublic_namecmp145
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryUSC
Corresponding_transgeneWBTransgene00026135
StatusLive
DescriptionPhenotypeWBPhenotype:0000306Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
Remark"PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. " "the in vivo dynamics of PGL-1 foci are drastically altered when tagged with mKate2 or mTagBFP2 and appear to be more solid or aggregate-like than when tagged with GFP." "PGL-1::GFP condensates in embryos are observed to dissolve at temperature shifts to 34C for 1 minute (Putnam et al., 2019). In adult germlines, evidence of P granule de-mixing can be seen by 3 hours at 34C ,where PGL-1::GFP foci are faint and fluorescent signal is heavily cytoplasmic (D, top row). However, this same temperature shift results in large bright, abnormal foci and low cytoplasmic signal in both PGL-1::mKate2 and PGL-1::mTagBFP2 (D, middle row, bottom row). The majority of these foci appear detached from the nuclear periphery and large, round, abnormal aggregates are also observed in the syncytial gonad rachis (not shown)."Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
"PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. " "the in vivo dynamics of PGL-1 foci are drastically altered when tagged with mKate2 or mTagBFP2 and appear to be more solid or aggregate-like than when tagged with GFP." " PGL1::GFP foci in the pachytene are disrupted 5 hours post-microinjection of -amanitin, a potent transcriptional inhibitor (E, top row). PGL-1::mKate2and PGL-1::mTagBFP2 both fail to dissolve, and instead form bright puncta that are abnormal compared to control foci (E, middle row, bottom row)."Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
ImageWBPicture0000014916Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004324Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005175PATO:0000460Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive34 degCPaper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00042744Paper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
Genotypepgl-1(cmp145[pgl-1::mKate2::Lox2272::3xMyc]) IVPaper_evidenceWBPaper00057192
Curator_confirmedWBPerson712
ReferenceWBPaper00057192
RemarkCreated in the nameserver by Karen Yook
WBPaper00057192 pgl-1 [pgl-1::mKate2::Lox2272::3xMyc]) IV
MethodAllele