WormBase Tree Display for Variation: WBVar02153018
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WBVar02153018 | Name | Public_name | ot1025 | |
---|---|---|---|---|
Sequence_details | SeqStatus | Pending_curation | ||
Origin | Species | Caenorhabditis elegans | ||
Laboratory | OH | |||
Status | Live | |||
Possibly_affects | WBGene00006756 | Paper_evidence | WBPaper00059740 | |
Remark | CGC_name unc-17 | |||
Reference | WBPaper00059740 | |||
Remark | [2020-06-09T02:38:31.216Z WBPerson712] New Variation: WBPaper00059740 ot1025 -The unc-17 regulatory allele ot1025 was generated using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 genome engineering, as described previously (Dokshin et al. 2018). The E3 motif was replaced with a PmeI restriction site with the following oligo repair template: tttttctgtctcaaaacggagctcgagcccatgacgtgtGTTTAAACtccgcccaattccttca (capital letters indicate the introduced PmeI site replacing the E3 motif). | Curator_confirmed | WBPerson712 | |
Method | Allele |