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WormBase Tree Display for Variation: WBVar02153018

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Name Class

WBVar02153018NamePublic_nameot1025
Sequence_detailsSeqStatusPending_curation
OriginSpeciesCaenorhabditis elegans
LaboratoryOH
StatusLive
Possibly_affectsWBGene00006756Paper_evidenceWBPaper00059740
RemarkCGC_name unc-17
ReferenceWBPaper00059740
Remark[2020-06-09T02:38:31.216Z WBPerson712] New Variation: WBPaper00059740 ot1025 -The unc-17 regulatory allele ot1025 was generated using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 genome engineering, as described previously (Dokshin et al. 2018). The E3 motif was replaced with a PmeI restriction site with the following oligo repair template: tttttctgtctcaaaacggagctcgagcccatgacgtgtGTTTAAACtccgcccaattccttca (capital letters indicate the introduced PmeI site replacing the E3 motif).Curator_confirmedWBPerson712
MethodAllele