hlh-25 encodes a REF-1-like protein with two bHLH domains that partially represses lag-2 transcription in ABa descendants (in response to a Notch signal, redundantly with HLH-27); lag-2 repression by HLH-25 may require UNC-37; HLH-25's paralogs include HLH-26 through HLH-29 and (more distantly) REF-1; hlh-25 is transcribed in the early MS lineage, has clustered binding sites for MED-1/2 in its promoter region, can be activated by ectopic MED-1, and loses activity in MED-1/2-deficient genetic backgrounds; HLH-25 is expressed in ABp granddaughters ~25 minutes after ABp first contacts an APX-1-expressing cell, and in ABa descendants after a second Notch interaction; HLH-25 is also expressed in four EMS granddaughters (MSaa, MSap, MSpa, MSpp) beginning at the 24-cell stage; ectopic HLH-25 can induce body muscle development in skn-1(RNAi);pal-1(RNAi) embryos that would otherwise lack muscle; HLH-25 is required for embryonic viability in mass RNAi assays.
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including dauer exit; determination of adult lifespan; and negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Expressed in several structures, including MSpa; anal depressor muscle; body wall musculature; intestinal cell; and neurons.
Map position created from combination of previous interpolated map position (based on known location of sequence) and allele information. Therefore this is not a genetic map position based on recombination frequencies or genetic experiments. This was done on advice of the CGC.