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WormBase Tree Display for Variation: WBVar00091812

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Name Class

WBVar00091812NamePublic_nameok525
Other_nameC12D8.10b.1:c.762+77_1249del
C12D8.10a.1:c.762+77_1234del
HGVSgCHROMOSOME_V:g.10250753_10252003del
Sequence_detailsSMapS_parentSequenceC12D8
Flanking_sequencesacttttgtttaaattttaaccccagcacgtGCAAGTTGAGTCAAGAAGCTAGAACTTTGC
Mapping_targetC12D8
Type_of_mutationDeletion
PCR_productOK525_external
OK525_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00031473
Component_of_genotypeWBGenotype00000019
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00000102
TranscriptC12D8.10c.1VEP_consequencesplice_acceptor_variant,coding_sequence_variant,3_prime_UTR_variant,intron_variant
VEP_impactHIGH
Intron_number5/5
Exon_number6/6
C12D8.10b.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScC12D8.10b.1:c.762+77_1249del
cDNA_position?-1300
CDS_position?-1249
Protein_position?-417
Intron_number6-8/10
Exon_number7-9/11
C12D8.10a.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScC12D8.10a.1:c.762+77_1234del
cDNA_position?-1283
CDS_position?-1234
Protein_position?-412
Intron_number6-8/10
Exon_number7-9/11
Interactor (17)
IsolationMutagenUV/TMP
DescriptionPhenotypeWBPhenotype:0000061Paper_evidenceWBPaper00036474
WBPaper00045812
Curator_confirmedWBPerson2987
Remark"Both akt-1(ok525) and akt-2(ok393) mutants showed a reproducible increase in lifespan relative to wild type (Fig 3g and Supplementary Table 1)."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
"Mutation in akt-2 exhibited greater lifespan extension of daf-16(mgDf50); daf-16a transgenic animals than a mutation in akt-1 (Fig 3h and Supplementary Table 1)."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
"... consistent with the DAF-16 nuclear translocation data, mutation in akt-1 resulted in a larger increase in lifespan of daf-16(mgDf50); daf-16d/f transgenic animals when compared with a mutation in akt-2 (Fig 3i and Supplementary Table 1)."Paper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
Table S1Paper_evidenceWBPaper00045812
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-16(mgDf50); DAF-16a::GFPPaper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
daf-16(mgDf50); DAF-16d/f::GFPPaper_evidenceWBPaper00036474
Curator_confirmedWBPerson2987
WBPhenotype:0000136Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkThe levels of both egl-1 and ced-13 transcripts are higher in akt-1 loss-of-function mutants following IR treatmentPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentIR treatmentPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0000731Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Remarkakt-1 loss-of-function mutants exhibited increased sensitivity to DNA-damage-induced germ-cell apoptosis 12, 24, and 36 hr after irradiationPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006796PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentTreated with 60 Gy IRPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001184Paper_evidenceWBPaper00035277
Curator_confirmedWBPerson2987
RemarkFigure S13d,ePaper_evidenceWBPaper00035277
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAnimals were stained with Nile red to assess intestinal fat contentPaper_evidenceWBPaper00035277
Curator_confirmedWBPerson2987
WBPhenotype:0001781Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkENU caused a significant increase in germline apoptosis in akt-1 and akt-2 loss of- function mutantsPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006796PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentTreated with 5mM ENUPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001817Paper_evidenceWBPaper00028460
Curator_confirmedWBPerson1918
RemarkAnimals are defective in learned avoidance of salt concentrations encountered under starvation conditions.Paper_evidenceWBPaper00028460
Curator_confirmedWBPerson1918
WBPhenotype:0001999Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkThe integration of signals for attraction to diacetyl (100x dilute) and avoidance from copper (100 millimolar) was impaired in akt-1(ok525) insulin-like signaling pathway mutants, resulting in more animals crossing the copper barrier to get to the diacetyl spot than in wild type controls (Figure 1C)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBMol:00002819Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0002268Paper_evidenceWBPaper00035277
Curator_confirmedWBPerson2987
RemarkFigure S13b,cPaper_evidenceWBPaper00035277
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAnimals were stained with Mitotracker Deep Red 633 to visualize intestinal mitochondriaPaper_evidenceWBPaper00035277
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000436Paper_evidenceWBPaper00028886
Curator_confirmedWBPerson48
RemarkLocalization of the synaptic protein SNB-1 is normal, based on transgene analysis with a reporter.Paper_evidenceWBPaper00028886
Curator_confirmedWBPerson48
WBPhenotype:0000481Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals did not display an abnormal aversion response to copper, compared to wild type animals (Figure 2B)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0000680Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals displayed a wild type response to 1 millimolar aldicarbPaper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00003650Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0000730Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkNone of the akt mutants affected developmental apoptosisPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0000741Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkGermline cell-cycle arrest was not altered in either akt-1 gain-of-function or loss-of-function mutantsPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0000885Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkNone of the akt mutants affected the engulfment rates of the germ-cell corpsesPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001470Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutants exhibited no change in chemotaxis towards diacetyl, compared to wild type controls (Figure 2A)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002819Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0004023Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals exhibit a wild type frequency of body bends (Figure 3A)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
ReferenceWBPaper00037970
WBPaper00028886
WBPaper00029085
WBPaper00028460
WBPaper00036474
WBPaper00035277
WBPaper00045812
WBPaper00065706
RemarkLast updated on 29 Nov 2002
Sequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele