Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00091951

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00091951EvidencePaper_evidenceWBPaper00032009
NamePublic_nameok667
HGVSg
Sequence_detailsSMapS_parentSequenceT03G6
Flanking_sequencesaaaaatcttgaattctagctatcgaaaaaaaaaatttaaactttatgaatctattgattt
Mapping_targetT03G6
Type_of_mutationInsertionAAAGGTNCCCN
Deletion
PCR_productok667_external
ok667_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00031553
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00003630
TranscriptT03G6.2c.1VEP_consequenceintron_variant
VEP_impactMODIFIER
Intron_number2/13
T03G6.2b.1VEP_consequenceintron_variant
VEP_impactMODIFIER
Intron_number2/13
InteractorWBInteraction000503695
IsolationMutagenUV/TMP
GeneticsMapping_dataIn_multi_point4787
DescriptionPhenotypeWBPhenotype:0000112Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkSeveral major peaks differed in a comparison of complete chromatograms between control and mutant animals; 50 major peaks were more abundant in control fractions, while 10 major peaks were more abundant in mutant animals.Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
WBPhenotype:0000119Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkMYO-3 levels are elevated relative to actin when compared to wild type animals. Levels of protein were assayed by Western blot with additional densitometric analysis.Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000023PATO:0000460Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
WBPhenotype:0000134Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkExpression levels of muscle genes, unc-95, unc-54, unc-60, unc-97, unc-98, unc-89 and myo-3 were not significantly altered. Levels of other muscle genes, unc-52, myo-2 and act-1 were significantly reduced compared to levels in N2 worms. Levels of myo-1 was mildly reduced.Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000023PATO:0000460Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkLow culture temperature dramatically increases the proportion of severely affected larva (n=4291).Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000023PATO:0000460Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitive16Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Phenotype_assayTemperature16, 20, 24Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
WBPhenotype:0001072Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkAnimals exhibit a progressively elevated penetrance of phenotypes as the food supply is restricted (n=3044).Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000023PATO:0000460Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitive16Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Phenotype_assayTreatmentTo test the effects of food restriction, bacterial suspensions were transferred on plates with the increasing volume so that plates with the following bacterial amounts were prepared: None, 3.3 x 105, 3.3 x 106, 3.3 x 107, and 1.65 x 109. Plates were irradiated on UV transluminator for 2 times 10 min each prior to inoculation of synchronized L1 larvae.Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Temperature16, 20, 24Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
WBPhenotype:0001569Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkAnimals exhibit severely disrupted myosin fibrils as detected by MYO-3 immunocytochemistry.Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
PenetranceIncompletePaper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000024PATO:0000460Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Temperature_sensitiveCold_sensitive16Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
Phenotype_assayTemperature16, 20, 24Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
WBPhenotype:0001644Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
RemarkMYO-3 elution profile is altered.Paper_evidenceWBPaper00032009
Curator_confirmedWBPerson712
ReferenceWBPaper00032009
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele