WormBase Tree Display for Variation: WBVar00094701

WBVar00094701 Evidence: Paper_evidence: WBPaper00004544
  Name: Public_name: or207
    Other_name: or207ts
      B0207.4.1:c.794C>T
      CE24761:p.Pro265Leu
      B0207.4.2:c.794C>T
    HGVSg: CHROMOSOME_I:g.5938465C>T
  Sequence_details: SMap: S_parent: Sequence: B0207
    Flanking_sequences: atttgattggaagattgcttgtcgttgatc caaggctcgctgtacgcttgaacaggtttg
    Mapping_target: B0207
    Type_of_mutation: Substitution: c t
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00007296
      WBStrain00007300
    Laboratory: EU
    Status: Live
  Affects: Gene: WBGene00000099
    Transcript: B0207.4.1 (12)
      B0207.4.2 (12)
    Interactor: WBInteraction000050631
      WBInteraction000050632
      WBInteraction000050633
      WBInteraction000051383
      WBInteraction000051730
      WBInteraction000557582
      WBInteraction000557584
      WBInteraction000557585
      WBInteraction000557586
  Genetics: Interpolated_map_position: I 0.482562
  Description: Phenotype: WBPhenotype:0000050 Paper_evidence: WBPaper00032264
          WBPaper00032269
        Curator_confirmed: WBPerson2021
          WBPerson712
        Remark: When shifted to restrictive temperatures, air-2(or207ts) hermaphrodites produce dead polyploid one-cell embryos Paper_evidence: WBPaper00032264
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000003 PATO:0000460 Paper_evidence: WBPaper00032264
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
            20 Paper_evidence: WBPaper00032269
              Curator_confirmed: WBPerson712
      WBPhenotype:0000436 Paper_evidence: WBPaper00032264
        Curator_confirmed: WBPerson2021
        Remark: The mutant AIR-2 protein fails to dissociate from anaphase chromosomes and localize to the spindle midzone and midbody Paper_evidence: WBPaper00032264
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Staining with DAPI and anti- AIR-2 antibody Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001351 Paper_evidence: WBPaper00032264
        Curator_confirmed: WBPerson2021
        Remark: Aurora A and B autophosphorylation and kinase activation is reduced in air-2 mutants Paper_evidence: WBPaper00032264
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Staining with phospho-specific pAurora antibody Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001378 Paper_evidence: WBPaper00032264
        Curator_confirmed: WBPerson2021
        Remark: Gross defects in mitotic chromosome segregation Paper_evidence: WBPaper00032264
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Staining with DAPI and anti-tubulin antibody Paper_evidence: WBPaper00032264
              Curator_confirmed: WBPerson2021
      WBPhenotype:0002408 Paper_evidence: WBPaper00042416
        Curator_confirmed: WBPerson3249
        EQ_annotations: GO_term: GO:0000910 PATO:0000460 Paper_evidence: WBPaper00042416
                Curator_confirmed: WBPerson3249
    Phenotype_not_observed: WBPhenotype:0000313 Paper_evidence: WBPaper00032269
        Curator_confirmed: WBPerson712
        Remark: Cytological analysis of DAPI-stained germlines from mutant worms revealed six bivalents like that observed in wild type. Paper_evidence: WBPaper00032269
            Curator_confirmed: WBPerson712
        Temperature_sensitive: Heat_sensitive: Paper_evidence: WBPaper00032269
              Curator_confirmed: WBPerson712
      WBPhenotype:0001175 Paper_evidence: WBPaper00032269
        Curator_confirmed: WBPerson712
        Phenotype_assay: Temperature: 20 Paper_evidence: WBPaper00032269
              Curator_confirmed: WBPerson712
  Reference: WBPaper00032264
    WBPaper00032269
    WBPaper00042416
    WBPaper00045580
  Remark: Codon 265 of the air-2 gene was mutated from CCC to CTC in or207ts [030409 ck1]
  Method: Substitution_allele