WormBase Tree Display for Variation: WBVar00143608

WBVar00143608 Evidence: Paper_evidence: WBPaper00026941
  Name: Public_name: e928
  Sequence_details: Mapping_target: ZK897
    Type_of_mutation: Deletion
    SeqStatus: Pending_curation
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00004215
      WBStrain00004554
      WBStrain00004557
      WBStrain00004558
      WBStrain00005458
      WBStrain00005465
      WBStrain00005466
      WBStrain00005475
      WBStrain00056258
      WBStrain00057281
      WBStrain00057283
    Laboratory: CB
      ZAS
      NCA
    Status: Live
  Affects: Gene: WBGene00006767
    Interactor: WBInteraction000052071
      WBInteraction000052107
      WBInteraction000052182
      WBInteraction000052339
      WBInteraction000504821
      WBInteraction000534853
      WBInteraction000537564
  Genetics: Mapping_data: In_2_point: 4247
        4450
        5527
      In_multi_point: 1263
        1535
        1537
        1750
        1876
        1922
        1924
        1926
  Description: Phenotype: WBPhenotype:0000017 Paper_evidence: WBPaper00006029
          WBPaper00004310
        Curator_confirmed: WBPerson712
          WBPerson2021
        Remark: Aldicarb resistance was most apparent at 15C but was almost completely rescued at 25C. Paper_evidence: WBPaper00006029
            Curator_confirmed: WBPerson712
          unc-31 mutants are strongly resistant to aldicarb Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00006029
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003650 Paper_evidence: WBPaper00006029
                WBPaper00004310
              Curator_confirmed: WBPerson712
                WBPerson2021
        Temperature_sensitive: Cold_sensitive: 15 Paper_evidence: WBPaper00006029
              Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were monitored for paralysis over time in the presence of 1mM alicarb. Paper_evidence: WBPaper00006029
              Curator_confirmed: WBPerson712
          Temperature: 15, 25 Paper_evidence: WBPaper00006029
              Curator_confirmed: WBPerson712
      WBPhenotype:0000147 Paper_evidence: WBPaper00048637
        Curator_confirmed: WBPerson712
        Remark: Mutants have an increased L1 starvation survival rate. Paper_evidence: WBPaper00048637
            Curator_confirmed: WBPerson712
        EQ_annotations: Life_stage: WBls:0000024 PATO:0000460 Paper_evidence: WBPaper00048637
                Curator_confirmed: WBPerson712
      WBPhenotype:0000204 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: aBoc is only executed ~50% of the time in each cycle. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: 50 Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
        Semi_dominant: Paper_evidence: WBPaper00030754
          Curator_confirmed: WBPerson712
        Haplo_insufficient: Paper_evidence: WBPaper00030754
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
      WBPhenotype:0000391 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: 75% of the time Emc precedes aBoc. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: 75 Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
        Recessive: Paper_evidence: WBPaper00030754
          Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
      WBPhenotype:0000397 Paper_evidence: WBPaper00032087
        Curator_confirmed: WBPerson2021
        Remark: Animals are essentially unresponsive to harsh physical stimulation Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000420 Paper_evidence: WBPaper00004310
        Curator_confirmed: WBPerson2021
        Remark: unc-31 mutants are hypersensitive to levamisole Paper_evidence: WBPaper00004310
            Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00004019 Paper_evidence: WBPaper00004310
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000637 Paper_evidence: WBPaper00042548
        Curator_confirmed: WBPerson237
        Remark: Figure 1, 2 and 3 Paper_evidence: WBPaper00042548
            Curator_confirmed: WBPerson237
      WBPhenotype:0000644 Paper_evidence: WBPaper00030754
          WBPaper00032087
        Curator_confirmed: WBPerson712
          WBPerson2021
        Remark: Animals removed from food move faster than animals on food. Animals starved for 5 hours and assayed on plates without food move more like WT animals. Paralysis is less in older adults. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
          Animals are nearly paralyzed Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00030754
              WBPaper00032087
            Curator_confirmed: WBPerson712
              WBPerson2021
      WBPhenotype:0000646 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: resembles e169, strongest allele Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0001278 Paper_evidence: WBPaper00050142
        Curator_confirmed: WBPerson2987
        Remark: "In our analyses, we found that unc-31(e928) mutation was able to suppress cell-non-autonomous UPRmt induction (Figure 4A)." Paper_evidence: WBPaper00050142
            Curator_confirmed: WBPerson2987
        EQ_annotations: GO_term: GO:0034514 PATO:0000460 Paper_evidence: WBPaper00050142
                Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: rmIs101 [rgef-1p::Q40::YFP]; zcIs13 [hsp-6p::GFP] Paper_evidence: WBPaper00050142
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001285 Paper_evidence: WBPaper00045598
        Curator_confirmed: WBPerson712
        Remark: Following exposure to 10% CO2, the pumping rate of the pharynx was significantly higher compared with that in wild-type animals exposed to the same concentration of CO2. Paper_evidence: WBPaper00045598
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003023 Paper_evidence: WBPaper00045598
              Curator_confirmed: WBPerson712
      WBPhenotype:0001410 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: As assayed by the absence of a signal on a Western blot probed with an antibody generated against aa 42-304 of UNC-31. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
      WBPhenotype:0001426 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: As assayed by ANF::GFP fluorescence accumulation in coelomocytes. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: EG3681 unc-13(e928)oxIs206[Paex-3:ANF::GFP] Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
      WBPhenotype:0001513 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: As assayed by regulated release of ANF::GFP from cultured neurons in depolarizing, Ba++ containing, buffer. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: tgIs5[Paex-3:ANF::GFP] unc-13(e928) Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
      WBPhenotype:0001897 Paper_evidence: WBPaper00032087
        Curator_confirmed: WBPerson2021
        Remark: Wild-type animals required about half as much light power as the unc-31 null mutants to maximize their responses to blue and blue-violet light. Wild type's peak locomotion rate in 720-W/mm<sup>2</sup> blue violet light was ~1.5-fold higher than the unc-31 null mutant. unc-31 null showed delayed response initiation and took about twice as long as wild-type for its response to decay to basal levels Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000146 Paper_evidence: WBPaper00032087
        Curator_confirmed: WBPerson2021
        Remark: Abrupt temperature increases did not impact locomotion rate in unc-31 mutants and wild-type. Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032087
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000157 Paper_evidence: WBPaper00031426
        Curator_confirmed: WBPerson712
        Remark: Animals exhibit a normal number of posterior body contractions/cycle (87/87). Paper_evidence: WBPaper00031426
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: The presence or absence of each muscle contraction was scored for 11 defecation cycles in day 1 adults (n 6). Paper_evidence: WBPaper00031426
              Curator_confirmed: WBPerson712
      WBPhenotype:0000306 Paper_evidence: WBPaper00042524
        Curator_confirmed: WBPerson2987
        Remark: "In contrast to a loss of unc-13, when the rab-3::xbp-1s strain was crossed with unc-31(e928), we found very little, if any, effect on distal UPR-ER activation or activation in the nervous system (Figures 7B and S6E)." Paper_evidence: WBPaper00042524
            Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: rab-3p::xbp-1s; hsp-4p::GFP Paper_evidence: WBPaper00042524
              Curator_confirmed: WBPerson2987
      WBPhenotype:0000436 Paper_evidence: WBPaper00028886
        Curator_confirmed: WBPerson48
        Remark: Localization of the synaptic protein SNB-1 is normal, based on expression analysis of SNB-1::VENUS. Paper_evidence: WBPaper00028886
            Curator_confirmed: WBPerson48
      WBPhenotype:0000623 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: Acute expression of UNC-31 in unc-31 null mutants after the completion of nervous system development fully rescues the defecation cycle defects in adult mutants. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: oxIs179[hsp:UNC-31]unc-13(e928) Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
      WBPhenotype:0000654 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: As assayed by uptake of FM-64 by cultured neurons in depolarization buffer. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: unc-13(e928) jsIs219 [synaptogyrin::GFP] Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
      WBPhenotype:0000679 Paper_evidence: WBPaper00030754
        Curator_confirmed: WBPerson712
        Remark: Localization of the pan-neuronal expressed preproANF::GFP is normal. Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00030754
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: tgIs5[Paex-3:ANF::GFP] unc-13(e928) Paper_evidence: WBPaper00030754
              Curator_confirmed: WBPerson712
      WBPhenotype:0001512 Paper_evidence: WBPaper00031992
        Curator_confirmed: WBPerson712
        Remark: ON-OFF asymmetry of ASEL and ASER was preserved in each of these animals. Paper_evidence: WBPaper00031992
            Curator_confirmed: WBPerson712
      WBPhenotype:0001616 Paper_evidence: WBPaper00024698
        Curator_confirmed: WBPerson712
        Remark: Trimethadione significantly extended life-span of mutant animals (N=55)(1 ind.exp.), although to a less extent than observed for WT. Paper_evidence: WBPaper00024698
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Lifespan analysis was performed on animals exposed to 4 mg/ml of the drug from fertilization until death. Animals were cultured on NGM plates containing the drug and seeded with OP50. Paper_evidence: WBPaper00024698
              Curator_confirmed: WBPerson712
          Temperature: 20C Paper_evidence: WBPaper00024698
              Curator_confirmed: WBPerson712
      WBPhenotype:0001617 Paper_evidence: WBPaper00024698
        Curator_confirmed: WBPerson712
        Remark: Ethosuximide significantly extended life-span of mutant animals (N=95)(3 ind.exp.), as it does for WT animals. Paper_evidence: WBPaper00024698
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Lifespan analysis was performed on animals exposed to 2 mg/ml of the drug from fertilization until death. Animals were cultured on NGM plates containing the drug and seeded with OP50. Paper_evidence: WBPaper00024698
              Curator_confirmed: WBPerson712
          Temperature: 20C Paper_evidence: WBPaper00024698
              Curator_confirmed: WBPerson712
      WBPhenotype:0001800 Paper_evidence: WBPaper00033094
        Curator_confirmed: WBPerson2021
        Remark: Normal increase in pnlp-29::GFP expression after infection. Paper_evidence: WBPaper00033094
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00033094
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: frIs7 [pnlp-29::GFP] Paper_evidence: WBPaper00033094
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001818 Paper_evidence: WBPaper00032221
        Curator_confirmed: WBPerson712
        Remark: HSNs showed robust and rhythmic activity. Paper_evidence: WBPaper00032221
            Curator_confirmed: WBPerson712
        Variation_effect: Null: Paper_evidence: WBPaper00032221
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006830 PATO:0000460 Paper_evidence: WBPaper00032221
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were assayed 24 hr after the late fourth larval (L4) stage at 20.5C 0.5C. Paper_evidence: WBPaper00032221
              Curator_confirmed: WBPerson712
          Genotype: Cameleon IjIs25[myo-3::YC2.0] Paper_evidence: WBPaper00032221
              Curator_confirmed: WBPerson712
      WBPhenotype:0001838 Paper_evidence: WBPaper00033094
        Curator_confirmed: WBPerson2021
        Remark: The PMA-induced upregulation of pnlp-29::GFP expression was normal in mutant worms Paper_evidence: WBPaper00033094
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00033094
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: frIs7 [pnlp-29::GFP] Paper_evidence: WBPaper00033094
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001840 Paper_evidence: WBPaper00033094
        Curator_confirmed: WBPerson2021
        Remark: Normal increase in pnlp-29::GFP expression after wounding Paper_evidence: WBPaper00033094
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00033094
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: frIs7 [pnlp-29::GFP] Paper_evidence: WBPaper00033094
              Curator_confirmed: WBPerson2021
  Reference: WBPaper00004310
    WBPaper00015419
    WBPaper00022874
    WBPaper00014052
    WBPaper00028886
    WBPaper00017299
    WBPaper00024698
    WBPaper00013729
    WBPaper00006029
    WBPaper00031426
    WBPaper00032221
    WBPaper00013858
    WBPaper00033094
    WBPaper00032087
    WBPaper00014022
    WBPaper00013780
    WBPaper00030754
    WBPaper00031992
    WBPaper00014119
    WBPaper00019207
    WBPaper00014366
    WBPaper00014028
    WBPaper00020943
    WBPaper00023305
    WBPaper00016727
    WBPaper00042548
    WBPaper00042524
    WBPaper00045598
    WBPaper00048637
    WBPaper00050142
    WBPaper00064979
    WBPaper00065802
    WBPaper00065754
    WBPaper00066037
  Remark: The region deleted by the unc-31(e928) mutation, includes the entire C-terminal half of UNC-31 (553-1260 of 1260 aa total). Paper_evidence: WBPaper00026941
  Method: Deletion_allele