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WormBase Tree Display for Variation: WBVar00145089

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Name Class

WBVar00145089EvidencePaper_evidenceWBPaper00030757
NamePublic_nameec1
Sequence_detailsMapping_targetE03A3
SeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00005209
LaboratoryFH
StatusLive
AffectsGeneWBGene00006811
InteractorWBInteraction000052184
WBInteraction000052190
WBInteraction000052193
WBInteraction000052198
WBInteraction000052203
WBInteraction000052204
WBInteraction000052205
WBInteraction000052321
WBInteraction000052323
WBInteraction000052354
DescriptionPhenotypeWBPhenotype:0000643Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
WBPhenotype:0000646Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
WBPhenotype:0000843Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RemarkMales are not as potent as unc-80 animals.Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
WBPhenotype:0001592Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RemarkFainter phenotype reported to authors by J.A. Lewis.Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
WBPhenotype:0001611Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RemarkAnimals have a lower EC50 (1.1 0.06 vol%) compared to N2 (3.2 0.06 vol%). By 2 vol% halothane, virtually all unc-79 animals were completely immobile. EC50 is the effective dose at which 50% of the animals are anesthetized.Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnesthetic response assay was performed as described in Morgan and Cascorbi, 1985. Briefly, agar plates of worms were placed in a sealed chamber, which was injected with halothane. Worms were observed through the lid of the chamber under a dissecting scope. When the animals stopped moving and assumed a straight posture they were considered anesthesized. The gas in the chamber was removed and its concentration determined with a gas chromatograph.Paper_evidenceWBPaper00000958
Curator_confirmedWBPerson712
WBPhenotype:0001612Paper_evidenceWBPaper00002358
Curator_confirmedWBPerson712
RemarkAnimals have a higher EC50 (1340 20mM) compared to N2 (1050 25 mM).Paper_evidenceWBPaper00002358
Curator_confirmedWBPerson712
Phenotype_assayTreatment50 animals were scored for mobility for 10 sec. at each concentration of ethanol after 5 min exposure. EC50's (concentration at which 50% of the animals were immobile for >10 sec) were determined from a dose response curve over a minimum of 12 concentrations (mM). Immobility was used an as endpoint. Upon removal from ethanol, immobility was reversible within 10-15 min. Treatment and scoring of anesthetic responses are described in Sedensky and Meneely, 1987; Morgan et al. , 1990; Morgan and Sedensky, 1994.Paper_evidenceWBPaper00002358
Curator_confirmedWBPerson712
Temperature22-24CPaper_evidenceWBPaper00002358
Curator_confirmedWBPerson712
ReferenceWBPaper00000958
WBPaper00002358
MethodAllele