WormBase Tree Display for Variation: WBVar00145089
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WBVar00145089 | Evidence | Paper_evidence | WBPaper00030757 | |||||
---|---|---|---|---|---|---|---|---|
Name | Public_name | ec1 | ||||||
Sequence_details | Mapping_target | E03A3 | ||||||
SeqStatus | Pending_curation | |||||||
Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Strain | WBStrain00005209 | |||||||
Laboratory | FH | |||||||
Status | Live | |||||||
Affects | Gene | WBGene00006811 | ||||||
Interactor | WBInteraction000052184 | |||||||
WBInteraction000052190 | ||||||||
WBInteraction000052193 | ||||||||
WBInteraction000052198 | ||||||||
WBInteraction000052203 | ||||||||
WBInteraction000052204 | ||||||||
WBInteraction000052205 | ||||||||
WBInteraction000052321 | ||||||||
WBInteraction000052323 | ||||||||
WBInteraction000052354 | ||||||||
Description | Phenotype | WBPhenotype:0000643 | Paper_evidence | WBPaper00000958 | ||||
Curator_confirmed | WBPerson712 | |||||||
Recessive | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000646 | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Recessive | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000843 | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Males are not as potent as unc-80 animals. | Paper_evidence | WBPaper00000958 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001592 | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Fainter phenotype reported to authors by J.A. Lewis. | Paper_evidence | WBPaper00000958 | |||||
Curator_confirmed | WBPerson712 | |||||||
Recessive | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001611 | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Animals have a lower EC50 (1.1 0.06 vol%) compared to N2 (3.2 0.06 vol%). By 2 vol% halothane, virtually all unc-79 animals were completely immobile. EC50 is the effective dose at which 50% of the animals are anesthetized. | Paper_evidence | WBPaper00000958 | |||||
Curator_confirmed | WBPerson712 | |||||||
Recessive | Paper_evidence | WBPaper00000958 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | Anesthetic response assay was performed as described in Morgan and Cascorbi, 1985. Briefly, agar plates of worms were placed in a sealed chamber, which was injected with halothane. Worms were observed through the lid of the chamber under a dissecting scope. When the animals stopped moving and assumed a straight posture they were considered anesthesized. The gas in the chamber was removed and its concentration determined with a gas chromatograph. | Paper_evidence | WBPaper00000958 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001612 | Paper_evidence | WBPaper00002358 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Animals have a higher EC50 (1340 20mM) compared to N2 (1050 25 mM). | Paper_evidence | WBPaper00002358 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | 50 animals were scored for mobility for 10 sec. at each concentration of ethanol after 5 min exposure. EC50's (concentration at which 50% of the animals were immobile for >10 sec) were determined from a dose response curve over a minimum of 12 concentrations (mM). Immobility was used an as endpoint. Upon removal from ethanol, immobility was reversible within 10-15 min. Treatment and scoring of anesthetic responses are described in Sedensky and Meneely, 1987; Morgan et al. , 1990; Morgan and Sedensky, 1994. | Paper_evidence | WBPaper00002358 | ||||
Curator_confirmed | WBPerson712 | |||||||
Temperature | 22-24C | Paper_evidence | WBPaper00002358 | |||||
Curator_confirmed | WBPerson712 | |||||||
Reference | WBPaper00000958 | |||||||
WBPaper00002358 | ||||||||
Method | Allele |