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WormBase Tree Display for Variation: WBVar00239352

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WBVar00239352	Evidence	Paper_evidence	WBPaper00032232
	Name	Public_name	pk924
	Sequence_details	Mapping_target	C46A5
		Type_of_mutation	Deletion
		SeqStatus	Pending_curation
	Variation_type	Allele
	Origin	Species	Caenorhabditis elegans
		Strain	WBStrain00040638
		Laboratory	NL
		Status	Live
	Affects	Gene	WBGene00001827
		Interactor	WBInteraction000518425
	Description	Phenotype	WBPhenotype:0000050	Paper_evidence	WBPaper00031249
				Curator_confirmed	WBPerson2645
				Remark	Figure 2	Paper_evidence	WBPaper00031249
						Curator_confirmed	WBPerson2645
				Temperature_sensitive	Cold_sensitive		Paper_evidence	WBPaper00031249
							Curator_confirmed	WBPerson2645
			WBPhenotype:0000061	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Animals exhibited up to 40% lifespan extension compared to wild-type animals. In general, animals lived longer than ok559 animals.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
			WBPhenotype:0000127	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Animals displayed a weak dauer exit defect.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Phenotype_assay	Treatment	Worms harboring the daf-2(e1370) mutation were incubated at the semi-nonpermissive temperature of 22 8C. Under this condition, the daf-2(ts) worms enter dauer for 3 d and then recover to become reproductive adults.	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
					Temperature	22.8	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
					Genotype	daf-2(e1370)	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
			WBPhenotype:0000461	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Worms were considerably more resistant to acute oxidative stress compared to wild-type worms, as assayed by treatment with a high-dose of paraquat.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Affected_by	Molecule	WBMol:00002747	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
				EQ_annotations	GO_term	GO:0034599	PATO:0000460	Paper_evidence	WBPaper00032232
								Curator_confirmed	WBPerson712
				Phenotype_assay	Treatment	Animals were assayed for survival in 200mM paraquat over time.	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
			WBPhenotype:0000717	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Expression of select DAF-16 target genes were significantly upregulated or downregulated compared to wild-type animals.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
			WBPhenotype:0001410	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Animals showed only background fluorescence when stained with affinity-purified polyclonal HCF-1 antibody in immunostaining assays (data not shown).	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Phenotype_assay	Genotype	sur-5::gfp	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
			WBPhenotype:0001654	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Worms were more resistant to heavy metal stress compared to wild-type worms, as assayed by exposure to cadmium.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Affected_by	Molecule	WBMol:00003022	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
				Phenotype_assay	Treatment	Animals were assayed for survival in 18mM CdCl2 at 24 and 32 hours.	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
		Phenotype_not_observed	WBPhenotype:0000146	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Worms behaved similar to wild-type animals throughout the time course of heat shock treatment at 35C.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Phenotype_assay	Treatment	Adult animals were shifted to 35C and monitored for survival.	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
					Temperature	35	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
			WBPhenotype:0000308	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Animals developed normally at all temperatures.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Phenotype_assay	Temperature	22, 25, 27	Paper_evidence	WBPaper00032232
							Curator_confirmed	WBPerson712
			WBPhenotype:0001182	Paper_evidence	WBPaper00032232
				Curator_confirmed	WBPerson712
				Remark	Animals did not show any substantial differences in fat storage from wild-type animals, as assayed by Nile Red staining.	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
				Variation_effect	Null	Paper_evidence	WBPaper00032232
						Curator_confirmed	WBPerson712
	Reference	WBPaper00032232
		WBPaper00031249
	Remark	pk924 is a large deletion	Paper_evidence	WBPaper00032232
		Manually curated Gene associations preserved as a text remark so that VEP is the canonical predictor of consequence: WBGene00001827 Frameshift
	Method	Deletion_allele