WormBase Tree Display for Construct: WBCnstr00008800
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| WBCnstr00008800 | Summary | [unc-25p::sng-1; ifb-2p::yfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006762 | ||
| Gene | WBGene00004912 | ||
| Construction_summary | The GABAergic rescue construct unc-25p::sng-1 was generated by first amplifying a HindIII/BamHI-flanked unc-25 promoter fragment with amplimers 10-135 (5=-TTA AGC TTG TCG ACT TTA AAA AAA TAT TTT TTT TTT CAG AAA ATA AGA AAA GCC G-3=) and 10-136 (5=-TGG ATC CGC GGC CGC TCT AGA TTT TGG CGG TGA ACT GAG C-3=) from genomic DNA. The promoter was then inserted into HindIII/BamHI-digested pVH20.01. Additionally, a 1866 bp NotI/BamHI-fragment encompassing the entire coding region of the sng-1 gene was amplified with amplimers 10-137 (5=-AAG CGG CCG CGG TAC CAT GGA GAA CGT GCG TGC TTA TG-3=) and 10-138 (5=-TAG GAT CCG ATA TCT CTA GAT TAA GAT ATC ATA ACC ATA TCC TTC CGA C-3=) from genomic DNA and placed into the unc-25p-containing vector. | ||
| Used_for | Transgene_construct | WBTransgene00009112 | |
| Reference | WBPaper00039786 |
