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WormBase Tree Display for RNAi: WBRNAi00096391

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Name Class

WBRNAi00096391HomolHomol_homolW06B3:RNAi
Y69A2AR:RNAi
Y17D7C:RNAi
W08F4:RNAi
Sequence_infoPCR_productsjj_W06B3.1
ExperimentLaboratoryGR
Date23 Feb 2012 00:00:00
StrainWBStrain00004861
TreatmentFor reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates.
Delivered_byBacterial_feeding
InhibitsPredicted_geneW06B3.1Inferred_automaticallyRNAi_primary
W08F4.12bInferred_automaticallyRNAi_primary
GeneWBGene00012295Inferred_automaticallyRNAi_primary
WBGene00021101Inferred_automaticallyRNAi_primary
WBGene00219343Inferred_automaticallyRNAi_primary
TranscriptW08F4.12cInferred_automaticallyRNAi_primary
W08F4.12dInferred_automaticallyRNAi_primary
Y17D7C.9Inferred_automaticallyRNAi_primary
W08F4.12b.1Inferred_automaticallyRNAi_primary
W06B3.1.2Inferred_automaticallyRNAi_primary
W08F4.12a.1Inferred_automaticallyRNAi_primary
W06B3.1.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00040984
Phenotype_not_observedWBPhenotype:0001236RemarkRNAi does not result in activation of the sod-3::GFP reporter gene
Remark(Table S2B) W06B3.1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
MethodRNAi