WormBase Tree Display for Variation: WBVar00054467
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WBVar00054467 | Name | Public_name | ct78 | ||||||
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Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00003961 | ||||||||
Laboratory | BW | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00000435 | |||||||
Interactor | WBInteraction000536128 | ||||||||
WBInteraction000536129 | |||||||||
WBInteraction000536130 | |||||||||
WBInteraction000536131 | |||||||||
WBInteraction000536132 | |||||||||
WBInteraction000536133 | |||||||||
WBInteraction000536134 | |||||||||
WBInteraction000536136 | |||||||||
WBInteraction000536138 | |||||||||
WBInteraction000536139 | |||||||||
Genetics | Mapping_data | In_multi_point | 1499 | ||||||
1500 | |||||||||
1501 | |||||||||
1502 | |||||||||
In_pos_neg_data (2) | |||||||||
Description | Phenotype | WBPhenotype:0000232 | Person_evidence | WBPerson261 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | defective CAN migrations | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006827 | PATO:0000460 | Person_evidence | WBPerson261 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000594 | Person_evidence | WBPerson261 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | migrations of cells other than CAN defective at low penetrance | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Penetrance | Low | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000816 | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "To investigate the role of CEH-10 in ALA neuron development, we first examined whether the ALA neuron was present in ceh-10(rf) and ceh-10 null mutant animals. In wild type, the ALA neuron is identifiable by its position in the dorsal head ganglion alongside the RID neuron, which is not the sister cell of ALA but also expresses CEH-10. By DIC optics the ALA and RID nuclei can be seen in a region flanked by hypodermal nuclei, along the dorsal midline (Fig 5A)... In ceh-10(ct78) reduction-of-function mutant animals, these neurons can be found in their wild-type positions in 75% of cases, and in the remaining animals a single cell can be found where the ALA and RID normally reside (Fig 5A,B). Our examination of ALA-specific reporters below reveals that approximately 90% of ceh-10(rf) animals possess an ALA neuron, and we infer that in the majority of cases in which only one of these neurons is detectable, it is the RID that is missing." | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003955 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0003938 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001224 | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "We then scored the extent of ALA axon migration and found that the ceh-10(rf) animals showed a ceh-17-like truncation of the ALA axons, albeit less severe (Fig 6B,C). While the ceh-17(lf) axon migration phenotype is similar when scored in either L1 or L2 stage animals, we found that a greater fraction of ceh-10(rf) ALA axons reach the tail by the L2 stage than at L1. This was surprising, as wild-type ALA axons complete their migration before hatching (Pujol et_al, 2000)." | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003955 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | unc-53:GFP | Paper_evidence | WBPaper00036308 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001278 | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "To examine the state of ALA differentiation in animals with reduced CEH-10 function, we examined the expression of panneuronal and ALA-specific reporter genes in ceh-10(ct78) mutant animals. We observed wild-type expression of the pan-neuronal reporters unc-119:YFP and rab-3:GFP in both the ALA and RID neurons (not shown). However, expression of the ALA-specific reporters flp-7:GFP and plc-3:YFP are impaired, and more at the L1 stage than at L4 (Fig 5C,D). Thus the ceh-10(rf) mutation impairs ALA-specific gene expression, but this defect is ameliorated as development proceeds." | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003955 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | flp-7:GFP | Paper_evidence | WBPaper00036308 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
plc-3:YFP | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001524 | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Another transcription factor expressed in ALA is the Q50 Prd-like protein CEH-10/Chx10, which has roles in the specification of the AIY, RMED, RID and CAN neurons (Forrester et_al, 1998). CAN function is required for viability, and animals lacking CEH-10 activity die as first-stage larvae (Forrester et_al, 1998). To examine CEH-10 in ALA function throughout development, we used a viable reduction-of-function mutation, ceh-10(ct78). We found the ceh-10(rf) mutation conferred partial EGF-resistance (Table 3), consistent with a role for CEH-10 in ALA function." | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003955 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | hs:LIN-3 | Paper_evidence | WBPaper00036308 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_not_observed | WBPhenotype:0000306 | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "To examine the state of ALA differentiation in animals with reduced CEH-10 function, we examined the expression of panneuronal and ALA-specific reporter genes in ceh-10(ct78) mutant animals. We observed wild-type expression of the pan-neuronal reporters unc-119:YFP and rab-3:GFP in both the ALA and RID neurons (not shown)." | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
"We also examined whether ceh-10(ct78) impairs the expression of ceh-17:GFP or ceh-14:GFP reporter genes in ALA, and found no difference from wild type, either at the L1 stage (Fig 5E) or later in development (not shown)." | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
"To determine whether CEH-10 and CEH-14 also play roles in ALA axon migration, we examined unc-53:GFP (pNP21; N. Pujol), which labels several neurons including ALA and the DA neurons of the ventral cord that extend commissural axons, marking body length (Stringham et al., 2002). In wild-type animals this reporter labels the ALA axons during the L1-L2 stages. We first examined unc-53:GFP in ALA in ceh-17(null) and ceh-10(rf) L1-L2 animals and found that expression of the reporter was not detectably impaired (Fig 6A)." | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003955 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
WBbt:0003938 | PATO:0000460 | Paper_evidence | WBPaper00036308 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | unc-119:YFP | Paper_evidence | WBPaper00036308 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
rab-3:GFP | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
ceh-17::GFP | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
ceh-14::GFP | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
unc-53:GFP | Paper_evidence | WBPaper00036308 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0001652 | Paper_evidence | WBPaper00032446 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Reference | WBPaper00032446 | ||||||||
WBPaper00013909 | |||||||||
WBPaper00036308 | |||||||||
WBPaper00016589 | |||||||||
Method | Allele |