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WormBase Tree Display for Variation: WBVar00088276

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Name Class

WBVar00088276EvidencePaper_evidenceWBPaper00004727
NamePublic_nameks5
Other_nameC40H5.5b.1:c.516+1G>A
C40H5.5a.1:c.600+1G>A
HGVSgCHROMOSOME_X:g.11766925C>T
Sequence_detailsSMapS_parentSequenceC40H5
Flanking_sequencesatttttcatcatgcgtgtttcgtatgtttttgagtttttaacaattttaaacaaattgtt
Mapping_targetC40H5
Type_of_mutationSubstitutionga
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00007506
WBStrain00052442
LaboratoryFK
StatusLive
AffectsGeneWBGene00006654
TranscriptC40H5.5b.1VEP_consequencesplice_donor_variant
VEP_impactHIGH
HGVScC40H5.5b.1:c.516+1G>A
Intron_number4/8
C40H5.5a.1VEP_consequencesplice_donor_variant
VEP_impactHIGH
HGVScC40H5.5a.1:c.600+1G>A
Intron_number5/10
InteractorWBInteraction000003413
WBInteraction000003414
WBInteraction000003415
WBInteraction000003416
WBInteraction000503643
WBInteraction000521604
WBInteraction000554842
WBInteraction000554843
WBInteraction000554844
WBInteraction000569170
GeneticsInterpolated_map_positionX6.71664
DescriptionPhenotypeWBPhenotype:0000997Paper_evidenceWBPaper00035614
Person_evidenceWBPerson261
Curator_confirmedWBPerson2021
WBPerson712
Remarkttx-3 mutants showed a strong tendency to migrate toward the cold area of the temperature gradient, regardless of the conditioning temperaturePaper_evidenceWBPaper00035614
Curator_confirmedWBPerson2021
Mutant animals cryophilic, almost completely defective in isothermal tracking.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001090Paper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
RemarkMutants are less viable than wild-type animals under conditions of heat stress. Reduced thermotolerance in these mutants is not due to a decline in hsf-1 levelsPaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentAnimals were exposed to a transient increase in temperature (30C or 34C for 15 min), and their heat shock response was measured as the total amount of hsp70 (C12C8.1) mRNA, 2 hours after heat shockPaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
WBPhenotype:0001273Paper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
RemarkMutations affecting the AFD or AIY neurons reduced heat shock-dependent accumulation of hsp70 (C12C8.1) mRNA at 30C and 34CPaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentAnimals were exposed to a transient increase in temperature (30C or 34C for 15 min), and their heat shock response was measured as the total amount of hsp70 (C12C8.1) mRNA, 2 hours after heat shockPaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
WBPhenotype:0001278Paper_evidenceWBPaper00031874
WBPaper00004727
Curator_confirmedWBPerson2021
WBPerson557
RemarkThe expression of hsp70 (C12C8.1)::GFP in gcy-8 and ttx-3 mutants was reduced in all somatic cells 2 hours after heat shock, unlike wild-typePaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
Animals were defective in autoregulation of a ttx-3prom::gfp reporter gene in the AIY interneuron. In Wildtype the expression is strong, in ks5 mutant animals the signal strength is clearly reduced, yet enough freely diffusible GFP protein is made in the cell to allow visualization of the axons.Paper_evidenceWBPaper00004727
Curator_confirmedWBPerson557
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentAnimals were exposed to a transient increase in temperature (30C or 34C for 15 min), and their heat shock response was measured as the total amount of hsp70 (C12C8.1) mRNA, 2 hours after heat shockPaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
GenotypeC12C8.1p::gfpPaper_evidenceWBPaper00031874
Curator_confirmedWBPerson2021
ttx-3prom::gfp (mgIs18)Paper_evidenceWBPaper00004727
Curator_confirmedWBPerson557
WBPhenotype:0001639Paper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
Remarkttx-3(AIY) mutants roamed twice less than wild type worms on foodPaper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
WBPhenotype:0002199Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Remark"In addition, ttx-3 mutants, which are defective in AIY function (Hobert et al., 1997), did not show the temperature-evoked suppression of RIA activity (Figure 5B)."Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006833PATO:0000460Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Phenotype_assayGenotypenjIs30[glr-3p::GCaMP3, glr-3p::TagRFP, ges-1p::nls-TagRFP] (V); Parent strain: IK1565Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
WBPhenotype:0002513Paper_evidenceWBPaper00004727
Curator_confirmedWBPerson557
RemarkThermotaxis defects mimic those seen upon laser ablation of the AIY interneurons.Paper_evidenceWBPaper00004727
Curator_confirmedWBPerson557
Phenotype_not_observedWBPhenotype:0000637Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkNo obvious abnormalities in dauer formation.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000648Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkNo obvious abnormalities in male mating.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000663Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkNo obvious abnormalities in osmotic avoidance.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001462Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkNo obvious abnormalities in chemotaxis to NaCl.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001661Paper_evidenceWBPaper00003760
Curator_confirmedWBPerson2021
RemarkAsymmetric expression in AWC was normalPaper_evidenceWBPaper00003760
Curator_confirmedWBPerson2021
Reference (16)
Remarkks5 is mutated at the splice donor site at exon 5 (G->A) [030414 ck1]
MethodSubstitution_allele