Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00088923

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00088923EvidencePaper_evidenceWBPaper00003903
NamePublic_namemg280
Other_nameZK1290.2a.1:c.86+68_854del
ZK1290.2b.1:c.-264+68_545del
HGVSgCHROMOSOME_II:g.7549511_7550816del
Sequence_detailsSMapS_parentSequenceZK1290
Flanking_sequencesccgttcaattttgaactgtaggagtgaactatactcggaaaataatattccgcaactaga
Mapping_targetZK1290
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00006655
WBStrain00007901
WBStrain00027519
WBStrain00049490
WBStrain00055118
WBStrain00055121
LaboratoryGR
MT
StatusLive
AffectsGeneWBGene00197293
WBGene00006600
TranscriptZK1290.2b.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
VEP_impactHIGH
HGVScZK1290.2b.1:c.-264+68_545del
cDNA_position?-875
CDS_position?-545
Protein_position?-182
Intron_number1-6/11
Exon_number2-7/12
ZK1290.2a.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScZK1290.2a.1:c.86+68_854del
cDNA_position?-877
CDS_position?-854
Protein_position?-285
Intron_number2-7/12
Exon_number3-8/13
ZK1290.18VEP_consequencenon_coding_transcript_exon_variant
VEP_impactMODIFIER
cDNA_position?-74
Exon_number1/1
InteractorWBInteraction000500144
WBInteraction000502728
WBInteraction000525337
WBInteraction000537565
WBInteraction000537566
WBInteraction000541577
GeneticsInterpolated_map_positionII0.504577
Mapping_dataIn_multi_point4733
DescriptionPhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00031438
Curator_confirmedWBPerson2369
WBPhenotype:0000010Paper_evidenceWBPaper00036766
Curator_confirmedWBPerson712
RemarkThe tph-1(mg280) mutant was fully sensitive to fluoxetine-induced paralysis. | Fluoxetine caused muscle relaxation in tph-1 mutants.Paper_evidenceWBPaper00036766
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00005058Paper_evidenceWBPaper00036766
Curator_confirmedWBPerson712
WBPhenotype:0000019Paper_evidenceWBPaper00032067
WBPaper00033168
WBPaper00045372
WBPaper00050740
Curator_confirmedWBPerson712
WBPerson2021
WBPerson2987
RemarkThe deficiency in endogenous serotonin caused a 40% reduction in pharynx pump rate.Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
The tph-1(mg280) strain shows a slight, but significant, reduction in the basal rate of pharyngeal pumping on food as compared to controlsPaper_evidenceWBPaper00033168
Curator_confirmedWBPerson2021
C. elegans tph-1 mutants exhibited significantly lower pharyngeal pumping rates than N2 control animals (A, N2 =3.46 0.19 Hz; tph-1 = 0.89 0.10 Hz; mean SEM; *** p < 0.0001, 2-tailed students t-test).Paper_evidenceWBPaper00050740
Curator_confirmedWBPerson712
ImageWBPicture0000013537Paper_evidenceWBPaper00050740
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0003681PATO:0000460Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
Phenotype_assayTreatmentPharyngeal pumping was measure in the presence of 100 mg/ml E. coli OP50 in M9 buffer, following a 2-hr fasting period.Paper_evidenceWBPaper00050740
Curator_confirmedWBPerson712
Temperature25Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
WBPhenotype:0000022Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0000039Paper_evidenceWBPaper00031694
WBPaper00048926
Curator_confirmedWBPerson8126
WBPerson11689
RemarkFig.4 partial reduction in lifespan extension compared to N2 wildtype upon reserpine treatmentPaper_evidenceWBPaper00031694
Curator_confirmedWBPerson8126
resistant to lifespan extension by serotonin antagonists (mianserin, etc)Paper_evidenceWBPaper00048926
Curator_confirmedWBPerson11689
Affected_byMoleculeWBMol:00002955Paper_evidenceWBPaper00031694
Curator_confirmedWBPerson8126
WBMol:00003509Paper_evidenceWBPaper00048926
Curator_confirmedWBPerson11689
WBPhenotype:0000061Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
RemarkIn the absence of drug, animals have a 4% extended life-span (24.4 days n=547) compared to N2 (23.5 days n=546).Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
Phenotype_assayTemperature20CPaper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
WBPhenotype:0000142Paper_evidenceWBPaper00048926
Curator_confirmedWBPerson11689
Remarkresistant to induction of stress resistance by serotonin antagonists (mianserin, etc)Paper_evidenceWBPaper00048926
Curator_confirmedWBPerson11689
Affected_byMoleculeWBMol:00003509Paper_evidenceWBPaper00048926
Curator_confirmedWBPerson11689
WBPhenotype:0000147Paper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
RemarkUnlike WT, well-fed and fasted tph-1 mutants failed to show significantly different behaviorsPaper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
WBPhenotype:0000353Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0000545Paper_evidenceWBPaper00028900
Curator_confirmedWBPerson557
WBPhenotype:0000577Paper_evidenceWBPaper00031915
Curator_confirmedWBPerson2021
RemarkOxygen consumption in mutants is reduced by 30% compared to wild-type controlsPaper_evidenceWBPaper00031915
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00031915
Curator_confirmedWBPerson2021
WBPhenotype:0000637Paper_evidenceWBPaper00042548
Curator_confirmedWBPerson237
RemarkFigure 1, 2 and 3Paper_evidenceWBPaper00042548
Curator_confirmedWBPerson237
WBPhenotype:0001013Paper_evidenceWBPaper00035315
Curator_confirmedWBPerson2021
Remarktph-1(mg280) exhibited enhanced susceptibility to PA14 relative to WT worms on the standard lawn assayPaper_evidenceWBPaper00035315
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00035315
Curator_confirmedWBPerson2021
WBPhenotype:0001042Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
RemarkVC neurons showed reduced activity, as demonstrated by a significantly low frequency of calcium spikes compared to wild-type animals.Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005304PATO:0000460Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested in low osmolarity conditions. Animals were assayed 24 hr after the late fourth larval (L4) stage at 20.5C 0.5C.Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
GenotypeCameleon IjIs25[myo-3::YC2.0]Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
WBPhenotype:0001183Paper_evidenceWBPaper00045372
Curator_confirmedWBPerson2987
WBPhenotype:0001265Paper_evidenceWBPaper00042154
Curator_confirmedWBPerson1455
RemarkIn free locomotion, head swing is highly irregular (Fig. 3), and head movement and body center movement are uncoordinated (Fig. 7).Paper_evidenceWBPaper00042154
Curator_confirmedWBPerson1455
WBPhenotype:0001278Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
Remark"Consistent with a role for serotonin in mediating the response to mitochondrial stress in the nervous system, mutations in tryptophan hydroxylase (tph-1), a key enzyme for serotonin synthesis, suppressed the induction of the hsp-6p::GFP reporter in the neuronal polyQ expressing animals (Figures 5A and 5B)."Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
"We found that loss of serotonin, by mutation of tph-1, blocked induction of the UPRmt in the spg-7 neuronal mutant model (Figures 6D and 6E)."Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
EQ_annotationsGO_termGO:0034514PATO:0000460Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
Phenotype_assayGenotypermIs101 [rgef-1p::Q40::YFP]; zcIs13 [hsp-6p::GFP]Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
rab-3p::Cas9; u6p::spg-7-sg; zcIs13 [hsp-6p::GFP]Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
WBPhenotype:0001444Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
RemarkAnimals exhibited defective gustatory plasticity of NaCl.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested for response to 25 mM NaCl after various pre-treatments: a 15-min wash in CTX buffer with or without 100 mM NaCl (liquid), or 30 min on a CTX plate with or without 100 mM NaCl, and in the presence or absence of bacteria, 500 mM glycerol, or 3 uL of benzaldehyde.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
WBPhenotype:0001462Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
RemarkAnimals showed significantly stronger chemotaxis to 1mM NaCl than wild-type animals.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested for response to 25 mM NaCl.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
WBPhenotype:0001602Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
RemarkMethiothepin did not extend the life-span of animals (-2% increase, n=63) as observed for N2 animals (28% increase, n=273).Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were exposed to 10uM methiothepin on day 1 of adulthood.Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
WBPhenotype:0001604Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
RemarkMianserin did not extend the life-span of animals (1% increase, n=314) as observed for N2 animals (31% increase, n=1180).Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were exposed to 50uM mianserin starting day 1 of adulthood.Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
WBPhenotype:0001639Paper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
RemarkSerotonin deficient tph-1 mutants were strongly defective in roamingPaper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
WBPhenotype:0001740Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
RemarkPharyngeal senescence was accelerated in these animals; however the normal pattern of morphological change was not altered compared with control animals, based on an aging model built on pharynx images from adults days 0-12. In this study fem-1(hc17) was the control.Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0003681PATO:0000460Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00032067
Curator_confirmedWBPerson712
WBPhenotype:0001818Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
RemarkHSN neurons showed hyperactivity, as demonstrated by increased calcium spike frequency compared to wild-type animals even in high osmolarity conditions.Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0006830PATO:0000460Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were tested in both low and osmolarity conditions. Animals were assayed 24 hr after the late fourth larval (L4) stage at 20.5C 0.5C.Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
GenotypeCameleon IjIs25[myo-3::YC2.0]Paper_evidenceWBPaper00032221
Curator_confirmedWBPerson712
WBPhenotype:0001842Paper_evidenceWBPaper00033168
Curator_confirmedWBPerson2021
Remarktph-1 animals did not show an overshoot in pharyngeal pumping following drug withdrawal, unlike N2Paper_evidenceWBPaper00033168
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentAnimals were treated with Clozapine (200 uM), fluphenazine (80 uM), or trifluoperazine (40 uM)Paper_evidenceWBPaper00033168
Curator_confirmedWBPerson2021
WBPhenotype:0001905Paper_evidenceWBPaper00028900
Curator_confirmedWBPerson557
WBPhenotype:0001911Paper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
RemarkWe confirmed that a loss-of-function mutation (mg280) of tph-1 caused a defect in axon regeneration in touch sensory posterior lateral microtubule (PLM) neurons. Furthermore, the mg280 and n4622 mutations of tph-1 were also defective in axon regeneration in GABA-releasing D-type motor neurons (Fig. 1d and Supplementary Table 2). Exogenous 5-HT treatment restored axon regeneration in tph-1mutants. These results suggest that 5-HT is generally required during axon regeneration. A single floxed copy of the tph-1 inserted gene was able to rescue the tph-1 defect in axon regeneration of D-type motorneurons. Expression of tph-1 in D neurons did indeed suppress the defect in axon regeneration of tph-1 mutantsPaper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
Rescued_by_transgeneWBTransgene00018707
WBTransgene00022921
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004929Paper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005190PATO:0000460Paper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
WBbt:0005490PATO:0000460Paper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
WBbt:0005270PATO:0000460Paper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
WBbt:0005303PATO:0000460Paper_evidenceWBPaper00049131
Curator_confirmedWBPerson712
WBPhenotype:0002284Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002288Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002293Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002296Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002300Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002303Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002309Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002313Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002319Paper_evidenceWBPaper00044094
Curator_confirmedWBPerson23309
WBPhenotype:0002322Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002325Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002328Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002335Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002346Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
WBPhenotype:0002378Paper_evidenceWBPaper00049802
Curator_confirmedWBPerson12526
WBPhenotype:0002506Paper_evidenceWBPaper00044624
Curator_confirmedWBPerson2706
Remarkmg280 clears labelled M. nematophilum infections faster than wild typePaper_evidenceWBPaper00044624
Curator_confirmedWBPerson2706
WBPhenotype:0004017Paper_evidenceWBPaper00042154
Curator_confirmedWBPerson1455
RemarkIn free locomotion, head swing is highly irregular (Fig. 3), and head movement and body center movement are uncoordinated (Fig. 7).Paper_evidenceWBPaper00042154
Curator_confirmedWBPerson1455
WBPhenotype:0004023Paper_evidenceWBPaper00043908
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000306Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
Remark"As a control for the specificity of serotonergic signaling to neuronal and cell-non-autonomous induction of the response, we exposed tph-1 mutant animals to cell-autonomous stressors, namely paraquat and cco-1 RNAi. These strains showed the same level of induction of hsp-6::GFP as controls, indicating that serotonin is required specifically for neuronal initiation of this response (Figure S4D)."Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002747Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
EQ_annotationsGO_termGO:0034514PATO:0000460Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
Phenotype_assayGenotypezcIs13 [hsp-6p::GFP]Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
zcIs13 [hsp-6p::GFP]; cco-1(RNAi)Paper_evidenceWBPaper00050142
Curator_confirmedWBPerson2987
WBPhenotype:0000631Paper_evidenceWBPaper00035198
Curator_confirmedWBPerson712
RemarkAnimals do not exhibit anterior convulsions when treated with pentylenetetrazole(PTZ).Paper_evidenceWBPaper00035198
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004251Paper_evidenceWBPaper00035198
Curator_confirmedWBPerson712
WBPhenotype:0001068Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
RemarkPre-exposure to mianserin blocked serotonin-induced egg laying as it does for N2 animals.Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were pre-exposed to 50uM mianserin.Paper_evidenceWBPaper00031241
Curator_confirmedWBPerson712
WBPhenotype:0001072Paper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
Remarktph-1 mutants displayed a wild type phenotype on non-eatable foodPaper_evidenceWBPaper00035327
Curator_confirmedWBPerson2021
WBPhenotype:0001101Paper_evidenceWBPaper00038382
Curator_confirmedWBPerson712
RemarkClozapine-induced increases in egg laying was present.Paper_evidenceWBPaper00038382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003634Paper_evidenceWBPaper00038382
Curator_confirmedWBPerson712
WBPhenotype:0001765Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
RemarkMutants defective in the synthesis and reception of nonessential excitatory neurotransmitters respond normally to CO2Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
Phenotype_assayTreatment10% CO2Paper_evidenceWBPaper00031936
Curator_confirmedWBPerson2021
WBPhenotype:0001780Paper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
RemarkMutants show normal butanone enhancementPaper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentPreexposure to 1:10 dilution of butanone and food. Animals were then subjected to odorant chemotaxis assays and/or selection assaysPaper_evidenceWBPaper00029060
Curator_confirmedWBPerson2021
WBPhenotype:0001817Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
RemarkAnimals exhibited enhanced-gustatory plasticity, i.e. strong avoidance to NaCl after starvation, similar to that observed for wild type.Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003571Paper_evidenceWBPaper00032335
Curator_confirmedWBPerson712
Disease_infoModels_diseaseDOID:809
DOID:1574
Models_disease_in_annotationWBDOannot00000681
WBDOannot00000714
ReferenceWBPaper00038382
WBPaper00043908
WBPaper00031936
WBPaper00035198
WBPaper00032221
WBPaper00036766
WBPaper00029060
WBPaper00035327
WBPaper00003903
WBPaper00018301
WBPaper00025798
WBPaper00010687
WBPaper00031241
WBPaper00031694
WBPaper00031915
WBPaper00032335
WBPaper00028900
WBPaper00031438
WBPaper00032067
WBPaper00033168
WBPaper00019518
WBPaper00025761
WBPaper00035315
WBPaper00019020
WBPaper00019156
WBPaper00024107
WBPaper00042548
WBPaper00042154
WBPaper00044094
WBPaper00044624
WBPaper00045372
WBPaper00048926
WBPaper00049131
WBPaper00050142
WBPaper00050740
WBPaper00049802
WBPaper00059382
WBPaper00060921
WBPaper00064927
WBPaper00065802
WBPaper00065715
RemarkVariation stub generated from the April 2021 NN VFP dataset.
Variation stub/paper connection generated from the May 2021 NN VFP dataset.
Created by WBPerson1983 from the NN_VFP_triage_pipeline
MethodDeletion_allele