WormBase Tree Display for Variation: WBVar00091661
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WBVar00091661 | Evidence | Person_evidence | WBPerson14358 | ||||||
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Name | Public_name | ok364 | |||||||
Other_name | C01B7.6.1:c.3291_5461del | ||||||||
CE06730:p.Ile1098AsnfsTer2 | |||||||||
HGVSg | CHROMOSOME_V:g.8813601_8815821del | ||||||||
Sequence_details | SMap | S_parent | Sequence | C01B7 | |||||
Flanking_sequences | agttgggctgtatggaggacgaggagaata | caattgaacgttgttatgggagcgaatggc | |||||||
Mapping_target | C01B7 | ||||||||
Type_of_mutation | Deletion | ||||||||
PCR_product | OK364_external | ||||||||
OK364_internal | |||||||||
SeqStatus | Sequenced | ||||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00031377 | ||||||||
Laboratory | RB | ||||||||
Person | WBPerson46 | ||||||||
KO_consortium_allele | |||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00004457 | |||||||
Transcript | C01B7.6.1 (11) | ||||||||
Interactor | WBInteraction000500068 | ||||||||
WBInteraction000500069 | |||||||||
WBInteraction000534756 | |||||||||
WBInteraction000537519 | |||||||||
Description | Phenotype | WBPhenotype:0000016 | Paper_evidence | WBPaper00031872 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | % animals paralyzed after 60 min on 1mM aldicarb was significantly higher than % N2 animals paralyzed. | Paper_evidence | WBPaper00031872 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00003650 | Paper_evidence | WBPaper00031872 | |||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0007833 | PATO:0001549 | Paper_evidence | WBPaper00031872 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000397 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | rpm-1 loss-of-function mutants have large defects in evoked responses to harsh touch. In wild-type animals, harsh touch resulted in robust reverse movement. In contrast, while all rpm-1 mutants responded to the harsh touch stimulus (n = 300), we observed a 40% reduction in reverse locomotion after harsh touch. | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Rescued_by_transgene | WBTransgene00021462 | ||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000420 | Paper_evidence | WBPaper00031872 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | % animals paralyzed after 60 min on 200mM levamisole was significantly higher than % N2 animals paralyzed under same conditions. | Paper_evidence | WBPaper00031872 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004019 | Paper_evidence | WBPaper00031872 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000646 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Analysis of the speed of locomotion showed that rpm-1 mutants moved 15-20% more slowly than wild-type animals during both forward and backward movement. | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Rescued_by_transgene | WBTransgene00021462 | ||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001933 | Paper_evidence | WBPaper00035070 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibit a temperature-sensitive Sam (synaptic vesicle tag abnormal in mechanosensory neuron) phenotype similar to mec-15 mutants. | Paper_evidence | WBPaper00035070 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005237 | PATO:0000460 | Paper_evidence | WBPaper00035070 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Temperature_sensitive | Heat_sensitive | Paper_evidence | WBPaper00035070 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001983 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | rpm-1 loss-of-function mutants have relatively mild abnormalities in exploratory locomotion. After removal from food, wild-type animals spent most of their time moving forward with small amounts of backward movement during reversals. rpm-1 mutants spent significantly less time moving forward compared with wild-type animals. The reduction in time spent moving forward was reflected by increased time spent moving backward, as opposed to staying stationary or performing compound/complex reorientations | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Rescued_by_transgene | WBTransgene00021462 | ||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002469 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | rpm-1 loss-of-function mutants have large defects in learning associated with tap habituation. RPM-1 function in the mechanosensory neurons affects habituation. Transgenic expression of RPM-1 in adult animals failed to rescue habituation defects, consistent with developmental defects in rpm-1 mutants resulting in impaired habituation. Genetic analysis showed that other regulators of neuronal development that function in the rpm-1 pathway (including glo-4, fsn-1, and dlk-1) also affected habituation. rpm-1 (lf) mutants had a significantly higher habituation level than wild-type animals; rpm-1 mutants fail to decrease their response to tap over time. rpm-1 mutants had a significant defect in habituation at all ages tested. Our results suggest that adult-specific expression of RPM-1 is not sufficient to rescue the habituation defects in rpm-1 mutants. | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Rescued_by_transgene | WBTransgene00021463 | ||||||||
WBTransgene00021462 | |||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000315 | Paper_evidence | WBPaper00035070 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals are touch sensitive. | Paper_evidence | WBPaper00035070 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000518 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | rpm-1 mutants developed and aged at a similar rate to wild-type animals as assessed by the onset of the L4 larval stage and egg-laying in synchronized populations (data not shown). | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000905 | Paper_evidence | WBPaper00035070 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibited normal cell-body morphology. | Paper_evidence | WBPaper00035070 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005237 | PATO:0000460 | Paper_evidence | WBPaper00035070 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001739 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | rpm-1 mutants developed and aged at a similar rate to wild-type animals as assessed by the onset of the L4 larval stage and egg-laying in synchronized populations (data not shown). | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002423 | Paper_evidence | WBPaper00049307 | |||||||
Curator_confirmed | WBPerson2987 | ||||||||
Remark | "Multiple MAP kinase pathways were not required for Neuro-Nmnat1(gcIs30[Neuro-m-nonN-Nmnat1]) hypoxic survival." (Figure S3c) | Paper_evidence | WBPaper00049307 | ||||||
Curator_confirmed | WBPerson2987 | ||||||||
EQ_annotations | GO_term | GO:0001666 | PATO:0000460 | Paper_evidence | WBPaper00049307 | ||||
Curator_confirmed | WBPerson2987 | ||||||||
Phenotype_assay | Genotype | gcIs30 [Neuro-m-nonN-Nmnat1] | Paper_evidence | WBPaper00049307 | |||||
Curator_confirmed | WBPerson2987 | ||||||||
WBPhenotype:0004027 | Paper_evidence | WBPaper00048707 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | rpm-1 (lf) mutants did not have a defect in the initial response totap stimulus. In fact, we found a very mild, but statistically significant,increase in the reversal probability in response to tap | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Variation_effect | Loss_of_function_undetermined_extent | Paper_evidence | WBPaper00048707 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00031872 | ||||||||
WBPaper00035070 | |||||||||
WBPaper00023967 | |||||||||
WBPaper00026122 | |||||||||
WBPaper00048707 | |||||||||
WBPaper00049307 | |||||||||
Remark | Last updated on 29 Nov 2002 | ||||||||
Allele sequenced by Stacey Edwards | Curator_confirmed | WBPerson2970 | |||||||
Sequenced by the C. elegans Gene Knockout Consortium | Paper_evidence | WBPaper00041807 | |||||||
Method | KO_consortium_allele |