Proteins that were modified by SUMO (smo-1). Using the strain RU86[smo-1(ok359); Is(psmo-1::smo-1gfp::3'UTRsmo-1] expressing HIS-GFP tagged SUMO, authors set up the purification of proteins modified by SUMO using immobilized metal ion affinity chromatography (IMAC).
Proteins were considered as SUMOylated if they were identified in at least 3 independent experiments, each protein identified with at least two peptides, FDR below 1% and not present in any of the control purifications from wild-type N2 worms or present with raw intensity greater than 10 times than in control purifications.